Evidence of photoinduced lipid hydroperoxidation in Langmuir monolayers containing Eosin Y.

Autor: Pereira LSA; School of Sciences, Humanities and Languages, São Paulo State University (UNESP), Assis, SP, 19806-900, Brazil., Camacho SA; School of Sciences, Humanities and Languages, São Paulo State University (UNESP), Assis, SP, 19806-900, Brazil., Malfatti-Gasperini AA; Brazilian Synchrotron Light Laboratory-LNLS, CNPEM, Rua Giuseppe Maximo Scolfaro, 10000, Campinas, SP 13083-070, Brazil., Jochelavicius K; IFSC, São Carlos Institute of Physics, University of São Paulo (USP), São Carlos, SP, 13566-590, Brazil., Nobre TM; IFSC, São Carlos Institute of Physics, University of São Paulo (USP), São Carlos, SP, 13566-590, Brazil., Oliveira ON Jr; IFSC, São Carlos Institute of Physics, University of São Paulo (USP), São Carlos, SP, 13566-590, Brazil., Aoki PHB; School of Sciences, Humanities and Languages, São Paulo State University (UNESP), Assis, SP, 19806-900, Brazil. Electronic address: pedro.aoki@unesp.br.
Jazyk: angličtina
Zdroj: Colloids and surfaces. B, Biointerfaces [Colloids Surf B Biointerfaces] 2018 Nov 01; Vol. 171, pp. 682-689. Date of Electronic Publication: 2018 Aug 06.
DOI: 10.1016/j.colsurfb.2018.08.002
Abstrakt: Photodynamic therapy (PDT) efficiency depends on many factors including the incorporation of the photosensitizer (PS) in cell membranes and possible lipid hydroperoxidation. In this study, we show that hydroperoxidation may be photoinduced when eosin Y is incorporated into Langmuir monolayers that serve as cell membrane models. This occurs for Langmuir monolayers of 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC) and 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC), which have unsaturation in their hydrophobic chains. In contrast, light irradiation had no effect on monolayers of saturated 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC). Evidence of hydroperoxidation was obtained from the area increase in eosin-containing DOPC and POPC monolayers upon irradiation, which was accompanied by a decrease in monolayer thickness according to grazing incidence X-ray off-specular scattering (GIXOS) data. Furthermore, the changes in polarization-modulated infrared reflection absorption spectroscopy (PM-IRRAS) induced by irradiation were consistent with hydroperoxide migration toward the lipid hydrophilic heads.. In summary, this combination of experimental methods allowed us to determine the effects of eosin Y interaction with cell membrane models under irradiation, which may be associated with the underlying mechanisms of eosin Y as photosensitizer in PDT.
(Copyright © 2018 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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