| Autor: |
Vincent GP; Department of Biology and Microbiology, South Dakota State University, Brookings, SD 57007, USA., Davis JK; Geospatial Sciences Center of Excellence, South Dakota State University, Brookings, SD 57007, USA., Wimberly MC; Geospatial Sciences Center of Excellence, South Dakota State University, Brookings, SD 57007, USA., Carlson CD; South Dakota Department of Health, Public Health Laboratory, Pierre, SD 57501, USA., Hildreth MB; Department of Biology and Microbiology, South Dakota State University, Brookings, SD 57007, USA.; Department of Veterinary and Biomedical Sciences, South Dakota State University, Brookings, SD 57007, USA. |
| Abstrakt: |
In 2016, we compared susceptibility to the insecticide, permethrin, between the West Nile virus vector, Culex tarsalis Coquillett, and a major nuisance mosquito, Aedes vexans (Meigen), using baseline diagnostic dose and time values determined using the CDC bottle bioassay protocol. Mosquitoes were collected in the wild in Brookings County, South Dakota, situated in the Northern Great Plains of the USA. The determined diagnostic dose and time were then used in 2017 to validate these measurements for the same 2 mosquito species, collected at a second location within Brookings County. The diagnostic dose was determined for multiple time periods and ranged from 27.0 µ g/ml at 60 min to 38.4 µ g/ml at 30 min. There was no significant difference detected in mortality rates between Cx. tarsalis and Ae. vexans for any diagnostic time and dose. For practical purposes, mosquitoes in 2017 were tested at 38 µ g/ml for 30 min; expected mortality rates were 93.38% for Cx. tarsalis and 94.93% for Ae. vexans. Actual 2017 mortality rates were 92.68% for Cx. tarsalis and 96.12% for Ae. vexans , validating the usefulness of this baseline at an additional location and year. |
