Identification of 170 New Long Noncoding RNAs in Schistosoma mansoni .

Autor: Oliveira VF; Departamento de Ciências Biológicas, Núcleo de Pesquisas em Ciências Biológicas, Universidade Federal de Ouro Preto, Morro do Cruzeiro, Ouro Preto, MG, Brazil., Moares LAG; Departamento de Computação, Laboratório de Computação de Sistemas Inteligentes, Universidade Federal de Ouro Preto, Morro do Cruzeiro, Ouro Preto, MG, Brazil., Mota EA; Departamento de Ciências Biológicas, Núcleo de Pesquisas em Ciências Biológicas, Universidade Federal de Ouro Preto, Morro do Cruzeiro, Ouro Preto, MG, Brazil., Jannotti-Passos LK; Centro de Pesquisas René Rachou, Fiocruz, Belo Horizonte, MG, Brazil., Coelho PMZ; Centro de Pesquisas René Rachou, Fiocruz, Belo Horizonte, MG, Brazil., Mattos ACA; Centro de Pesquisas René Rachou, Fiocruz, Belo Horizonte, MG, Brazil., Couto FFB; Centro de Pesquisas René Rachou, Fiocruz, Belo Horizonte, MG, Brazil., Caffrey BE; Max Planck Institute for Molecular Genetics, Ihnestr. 63-73, 14195 Berlin, Germany., Marsico A; Max Planck Institute for Molecular Genetics, Ihnestr. 63-73, 14195 Berlin, Germany.; Freie Universitaet Berlin, Arnimallee 14, 14195 Berlin, Germany., Guerra-Sá R; Departamento de Ciências Biológicas, Núcleo de Pesquisas em Ciências Biológicas, Universidade Federal de Ouro Preto, Morro do Cruzeiro, Ouro Preto, MG, Brazil.
Jazyk: angličtina
Zdroj: BioMed research international [Biomed Res Int] 2018 Jul 11; Vol. 2018, pp. 1264697. Date of Electronic Publication: 2018 Jul 11 (Print Publication: 2018).
DOI: 10.1155/2018/1264697
Abstrakt: Long noncoding RNAs (lncRNAs) are transcripts generally longer than 200 nucleotides with no or poor protein coding potential, and most of their functions are also poorly characterized. Recently, an increasing number of studies have shown that lncRNAs can be involved in various critical biological processes such as organism development or cancer progression. Little, however, is known about their effects in helminths parasites, such as Schistosoma mansoni . Here, we present a computational pipeline to identify and characterize lncRNAs from RNA-seq data with high confidence from S. mansoni adult worms. Through the utilization of different criteria such as genome localization, exon number, gene length, and stability, we identified 170 new putative lncRNAs. All novel S. mansoni lncRNAs have no conserved synteny including human and mouse. These closest protein coding genes were enriched in 10 significant Gene Ontology terms related to metabolism, transport, and biosynthesis. Fifteen putative lncRNAs showed differential expression, and three displayed sex-specific differential expressions in praziquantel sensitive and resistant adult worm couples. Together, our method can predict a set of novel lncRNAs from the RNA-seq data. Some lncRNAs are shown to be differentially expressed suggesting that those novel lncRNAs can be given high priority in further functional studies focused on praziquantel resistance.
Databáze: MEDLINE