| Autor: |
Cid-Hernández M; Departamento de Química, Centro Universitario de Ciencias Exactas e Ingenierías, Universidad de Guadalajara, Blvd. Marcelino García Barragán 1421, 44430 Guadalajara, Jalisco, Mexico., López Dellamary-Toral FA; Departamento de Madera, Celulosa y Papel, Universidad de Guadalajara, km 15.5 de la Carretera Guadalajara-Nogales, 45220 Zapopan, Jalisco, Mexico., González-Ortiz LJ; Departamento de Química, Centro Universitario de Ciencias Exactas e Ingenierías, Universidad de Guadalajara, Blvd. Marcelino García Barragán 1421, 44430 Guadalajara, Jalisco, Mexico., Sánchez-Peña MJ; Departamento de Química, Centro Universitario de Ciencias Exactas e Ingenierías, Universidad de Guadalajara, Blvd. Marcelino García Barragán 1421, 44430 Guadalajara, Jalisco, Mexico., Pacheco-Moisés FP; Departamento de Química, Centro Universitario de Ciencias Exactas e Ingenierías, Universidad de Guadalajara, Blvd. Marcelino García Barragán 1421, 44430 Guadalajara, Jalisco, Mexico. |
| Abstrakt: |
Spirulina platensis contains several biologically active compounds, some of them with antioxidant activity. Nevertheless, not all of these compounds have been identified to date. As a first step to achieving such identification, a methodology to perform two-dimensional thin layer chromatography bioautographies on silica gel thin layer chromatography plates was proposed. Starting with a reference binary system, 5 other binary systems were tested, in which the relative polarity was systematically increased. To further improve the separation behavior, a phase modifier (NH 4 OH) was used. The best separation results were obtained with the isopropyl alcohol/ethyl acetate/NH 4 OH ternary system. This experimental system allowed four well-resolved spots showing antioxidant activity as well as two additional areas with mixtures containing antioxidant compounds. Although the proposed methodology was designed with a specific application, it would be predictable that its field of use could be considerably greater, making the convenient modifications on the solvent polarity and "masking level" produced by the ammonium derivatives. |
