Label-free, spatially multiplexed SPR detection of immunoassays on a highly integrated centrifugal Lab-on-a-Disc platform.

Autor: Miyazaki CM; FPC@DCU - Fraunhofer Project Centre for Embedded Bioanalytical Systems at Dublin City University, School of Physical Sciences, Dublin City University, Ireland; Federal University of São Carlos, Sorocaba, SP, Brazil. Electronic address: celinamiyazaki@ufscar.br., Kinahan DJ; FPC@DCU - Fraunhofer Project Centre for Embedded Bioanalytical Systems at Dublin City University, School of Physical Sciences, Dublin City University, Ireland., Mishra R; FPC@DCU - Fraunhofer Project Centre for Embedded Bioanalytical Systems at Dublin City University, School of Physical Sciences, Dublin City University, Ireland., Mangwanya F; FPC@DCU - Fraunhofer Project Centre for Embedded Bioanalytical Systems at Dublin City University, School of Physical Sciences, Dublin City University, Ireland., Kilcawley N; FPC@DCU - Fraunhofer Project Centre for Embedded Bioanalytical Systems at Dublin City University, School of Physical Sciences, Dublin City University, Ireland., Ferreira M; Federal University of São Carlos, Sorocaba, SP, Brazil., Ducrée J; FPC@DCU - Fraunhofer Project Centre for Embedded Bioanalytical Systems at Dublin City University, School of Physical Sciences, Dublin City University, Ireland. Electronic address: jens.ducree@dcu.ie.
Jazyk: angličtina
Zdroj: Biosensors & bioelectronics [Biosens Bioelectron] 2018 Nov 15; Vol. 119, pp. 86-93. Date of Electronic Publication: 2018 Jul 29.
DOI: 10.1016/j.bios.2018.07.056
Abstrakt: As a direct, label-free method, Surface Plasmon Resonance (SPR) detection significantly reduces the needs for liquid handling and reagent storage compared to common enzyme-linked immunosorbent assays (ELISAs), thus enabling comprehensive multiplexing of bioassays on microfluidic sample-to-answer systems. This paper describes a highly integrated centrifugal Lab-on-a-Disc (LoaD) platform for automating the full process chain extending between plasma extraction and subsequent aliquoting to five parallelized reaction channels for quantitative SPR detection by an inexpensive smartphone camera. The entire, multi-step / multi-reagent operation completes within less than 1 h. While the emphasis of this work is on the fluidic automation and parallelization by previously introduced, very robust event-triggered valving and buoyancy-driven centripetal pumping schemes, we successfully implement an immunoglobulin G (IgG) assay; by specific functionalization of the detection surfaces, the same disc layout can readily be customised for immunoassays panels from whole blood.
(Copyright © 2018 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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