Effects of GnRH and the dual regulatory actions of GnIH in the pituitary explants and brain slices of Astyanax altiparanae males.

Autor: Branco GS; Aquaculture Center of São Paulo State University (CAUNESP), São Paulo State University (UNESP), Jaboticabal Campus, Jaboticabal, Brazil; Reproductive and Molecular Biology Group, Department of Morphology, Institute of Biosciences, São Paulo State University (UNESP), Botucatu Campus, Botucatu, Brazil., Melo AG; Reproductive and Molecular Biology Group, Department of Morphology, Institute of Biosciences, São Paulo State University (UNESP), Botucatu Campus, Botucatu, Brazil., Ricci JMB; Reproductive and Molecular Biology Group, Department of Morphology, Institute of Biosciences, São Paulo State University (UNESP), Botucatu Campus, Botucatu, Brazil., Digmayer M; Reproductive and Molecular Biology Group, Department of Morphology, Institute of Biosciences, São Paulo State University (UNESP), Botucatu Campus, Botucatu, Brazil., de Jesus LWO; Institute of Biological Sciences and Health, Federal University of Alagoas - A. C., Simões Campus, Maceió, Brazil., Habibi HR; Department of Biological Sciences, University of Calgary, Calgary, Canada., Nóbrega RH; Reproductive and Molecular Biology Group, Department of Morphology, Institute of Biosciences, São Paulo State University (UNESP), Botucatu Campus, Botucatu, Brazil. Electronic address: nobregarh@ibb.unesp.br.
Jazyk: angličtina
Zdroj: General and comparative endocrinology [Gen Comp Endocrinol] 2019 Mar 01; Vol. 273, pp. 209-217. Date of Electronic Publication: 2018 Aug 09.
DOI: 10.1016/j.ygcen.2018.08.006
Abstrakt: The pituitary gonadotropins, Fsh (follicle-stimulating hormone) and Lh (luteinizing hormone), regulate testicular development and functions in all vertebrates. At the pituitary, different signaling systems regulate the synthesis and secretion of the gonadotropins, such as the hypothalamic neuropeptides GnRH (gonadotropin-releasing hormone) and GnIH (gonadotropin-inhibitory hormone). While GnRH exerts stimulatory roles, the actions of GnIH remain controversial for many teleost species. Therefore, the aim of this study was to evaluate the in vitro effects of chicken GnRH2 (cGnRH2) and zebrafish GnIH-3 (zGnIH-3) on the male gonadotropin and GnRH system expression using pituitary explants and brain slices from a neotropical species with economical and ecological relevance, Astyanax altiparanae. Our results showed that in males, cGnRH2 increased fshb and lhb mRNA levels in the pituitary explants. Interestingly, zGnIH-3 has no effect on basal gonadotropin expression, however zGnIH-3 decreased the cGnRH2-induced fshb and lhb transcripts in male pituitary explants. In the male brain slices, zGnIH-3 showed stimulatory effects, increasing gnrh2 mRNA levels. Overall, our results suggested that GnIH seems to have dual regulatory actions on gonadotropin and GnRH2 expression of A. altiparanae males. This study provided basic information on endocrine regulation of A. altiparanae reproduction, and the obtained results will expand our knowledge, improving the reproductive management of this economically important freshwater species.
(Copyright © 2018 Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
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