QueE: A Radical SAM Enzyme Involved in the Biosynthesis of 7-Deazapurine Containing Natural Products.

Autor: Lewis JK; Department of Chemistry, University of Utah, Salt Lake City, UT, United States., Bruender NA; Department of Chemistry and Biochemistry, St. Cloud State University, St. Cloud, MN, United States., Bandarian V; Department of Chemistry, University of Utah, Salt Lake City, UT, United States. Electronic address: vahe@chem.utah.edu.
Jazyk: angličtina
Zdroj: Methods in enzymology [Methods Enzymol] 2018; Vol. 606, pp. 95-118. Date of Electronic Publication: 2018 Jul 13.
DOI: 10.1016/bs.mie.2018.05.001
Abstrakt: 7-Carboxy-7-deazaguanine (CDG) is a common intermediate in the biosynthesis of 7-deazapurine-containing natural products. The biosynthesis of CDG from GTP requires three enzymes: GTP cyclohydrolase I, 6-carboxy-5,6,7,8-tetrahydropterin (CPH 4 ) synthase, and CDG synthase (QueE). QueE is a member of the radical S-adenosyl-l-methionine (SAM) superfamily and catalyzes the SAM-dependent radical-mediated ring contraction of CPH 4 to generate CDG. This chapter focuses on methods to reconstitute the activity of QueE in vitro.
(© 2018 Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
Externí odkaz: