Effects of waterborne exposure to the antidepressant fluoxetine on swimming, shoaling and anxiety behaviours of the mosquitofish Gambusia holbrooki.

Autor: Meijide FJ; Laboratorio de Ecotoxicología Acuática, Departamento de Biodiversidad y Biología Experimental, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, C1428EGA Ciudad Autónoma de Buenos Aires, Argentina; Instituto de Biodiversidad y Biología Experimental y Aplicada, CONICET-UBA, Ciudad Autónoma de Buenos Aires, Argentina. Electronic address: fmeiji@bg.fcen.uba.ar., Da Cuña RH; Laboratorio de Ecotoxicología Acuática, Departamento de Biodiversidad y Biología Experimental, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, C1428EGA Ciudad Autónoma de Buenos Aires, Argentina; Instituto de Biodiversidad y Biología Experimental y Aplicada, CONICET-UBA, Ciudad Autónoma de Buenos Aires, Argentina., Prieto JP; Departamento de Neurofarmacología Experimental, Instituto de Investigaciones Biológicas Clemente Estable, Montevideo, Uruguay., Dorelle LS; Instituto de Biodiversidad y Biología Experimental y Aplicada, CONICET-UBA, Ciudad Autónoma de Buenos Aires, Argentina., Babay PA; Gerencia Química, Centro Atómico Constituyentes, Comisión Nacional de Energía Atómica, 1650 Buenos Aires, Argentina., Lo Nostro FL; Laboratorio de Ecotoxicología Acuática, Departamento de Biodiversidad y Biología Experimental, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, C1428EGA Ciudad Autónoma de Buenos Aires, Argentina; Instituto de Biodiversidad y Biología Experimental y Aplicada, CONICET-UBA, Ciudad Autónoma de Buenos Aires, Argentina.
Jazyk: angličtina
Zdroj: Ecotoxicology and environmental safety [Ecotoxicol Environ Saf] 2018 Nov 15; Vol. 163, pp. 646-655. Date of Electronic Publication: 2018 Aug 07.
DOI: 10.1016/j.ecoenv.2018.07.085
Abstrakt: Chemical pollution from pharmaceuticals is increasingly recognized as a major hazard to the aquatic biota. Among the wide variety of pharmaceuticals, fluoxetine (FLX) is one of the most widely prescribed antidepressants, and therefore, it is frequently identified in the aquatic environment. As FLX is designed to alter human behaviour and many physiological pathways are conserved across vertebrates, this drug may affect the behaviour of fish living in FLX-polluted environments. Here, we exposed groups of female mosquitofish Gambusia holbrooki to waterborne FLX for 14 days, under semi-static conditions with daily renewal of test solutions. Following exposure, we conducted a set of behavioural assays in individual fish, aimed at assessing the effects of FLX on their locomotor activity and behavioural responses. We found that FLX impaired swimming behaviour at high concentrations (25 μg/L and 50 μg/L) but not at low concentrations close to environmental levels (1 μg/L and 5 μg/L). When swimming activity was assessed 5 min after transfer of the focal fish to the testing tank, 50 μg/L FLX was the only concentration showing significant effects. However, when the same trials were performed 24 h later, 25 μg/L FLX turned out to be an effect concentration in addition to 50 μg/L. Interestingly, these concentrations would elicit fish plasma concentrations comprised within the range of human therapeutic doses. When subjected to a light/dark preference test, fish showed tendency to remain less time in the dark area at high FLX concentrations, thus suggesting an anti-anxiety response. Shoaling behaviour was not affected by FLX exposure. Our study contributes to the growing body of literature evaluating the effects of FLX on animal behaviour. Regarding the experimental design used in behavioural testing, our findings suggest that focal fish should be subjected to long habituation periods, namely of at least a few hours, in order to better assess the effects of drug exposure.
(Copyright © 2018 Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
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