Treatment with Trichilia catigua ethyl-acetate fraction improves healing and reduces oxidative stress in TNBS-induced colitis in rats.

Autor: Vicentini FA; Laboratory of Animal Histology, Department of Morphological Sciences, State University of Maringá, Av. Colombo 5790, 87020-900, Maringá, PR, Brazil. Electronic address: fernandovicentini89@hotmail.com., Barbosa MMC; Department of Biochemistry, State University of Maringá, Av. Colombo 5790, 87020-900, Maringá, PR, Brazil. Electronic address: mamorselli@gmail.com., Fortunato MC; Laboratory of Animal Histology, Department of Morphological Sciences, State University of Maringá, Av. Colombo 5790, 87020-900, Maringá, PR, Brazil. Electronic address: maisa1fortunato@gmail.com., Amado CAB; Laboratory of Inflammation, Department of Pharmacology and Therapeutics, State University of Maringá, Av. Colombo 5790, 87020-900, Maringá, PR, Brazil. Electronic address: cabamado@uem.br., Comar JF; Department of Biochemistry, State University of Maringá, Av. Colombo 5790, 87020-900, Maringá, PR, Brazil. Electronic address: jfcomar@uem.br., Longhini R; Laboratory of Pharmaceutical Biology, Palafito, Department of Pharmacy, State University of Maringá, Av. Colombo 5790, 87020-900, Maringá, PR, Brazil. Electronic address: relonghini@hotmail.com., de Mello JCP; Laboratory of Pharmaceutical Biology, Palafito, Department of Pharmacy, State University of Maringá, Av. Colombo 5790, 87020-900, Maringá, PR, Brazil. Electronic address: mello@uem.br., Natali MRM; Laboratory of Animal Histology, Department of Morphological Sciences, State University of Maringá, Av. Colombo 5790, 87020-900, Maringá, PR, Brazil. Electronic address: mrmnatali@gmail.com.
Jazyk: angličtina
Zdroj: Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie [Biomed Pharmacother] 2018 Nov; Vol. 107, pp. 194-202. Date of Electronic Publication: 2018 Aug 05.
DOI: 10.1016/j.biopha.2018.07.160
Abstrakt: Beverages containing Trichilia catigua are commonly employed in folk medicine. T. catigua bark extracts possess antioxidant, anti-inflammatory, and bactericidal properties. These properties suggest T. catigua bark extracts as a potential treatment for inflammatory bowel diseases (IBD). Using the 2,4,6-trinitrobenzenesulphonic acid (TNBS)-induced model of colitis in rats we evaluated the effect of an ethyl-acetate fraction (EAF) of T. catigua (200 mg/kg) administered by daily oral gavage or intrarectally at different time points after TNBS challenge. TNBS treatment evoked severe colonic inflammation after 24 h that persisted for 7 days, characterized by weight loss, high levels of myeloperoxidase activity, histological and macroscopic damage, and elevated index of oxidative stress in the blood. T. catigua EAF treatment prevented the oxidative stress within 24 h and enhanced tissue recovery observed at day 7, returning histological and macroscopic damage levels to that of the control group. TNBS treatment led to loss of myenteric neurons after 28 days. T. catigua EAF was unable to prevent the neuronal loss. Oral delivery of T. catigua EAF was more effective than intrarectal administration of the extract. In conclusion, T. catigua EAF treatment normalized oxidative stress parameters in blood and reduced the degree of acute inflammation in TNBS colitis.
(Copyright © 2018 Elsevier Masson SAS. All rights reserved.)
Databáze: MEDLINE
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