Cranberry-derived proanthocyanidins induce a differential transcriptomic response within Candida albicans urinary biofilms.

Autor: Sundararajan A; National Center for Genome Resources, Santa Fe, NM, United States of America., Rane HS; Section of Infectious Diseases, New Mexico VA Healthcare System, Albuquerque, NM, United States of America., Ramaraj T; National Center for Genome Resources, Santa Fe, NM, United States of America., Sena J; National Center for Genome Resources, Santa Fe, NM, United States of America., Howell AB; Marucci Center for Blueberry and Cranberry Research and Extension, Rutgers, The State University of New Jersey, Chatsworth, NJ, United States of America., Bernardo SM; Division of Infectious Diseases, University of New Mexico Health Science Center, Albuquerque, NM, United States of America., Schilkey FD; National Center for Genome Resources, Santa Fe, NM, United States of America., Lee SA; Section of Infectious Diseases, New Mexico VA Healthcare System, Albuquerque, NM, United States of America.; Division of Infectious Diseases, University of New Mexico Health Science Center, Albuquerque, NM, United States of America.
Jazyk: angličtina
Zdroj: PloS one [PLoS One] 2018 Aug 08; Vol. 13 (8), pp. e0201969. Date of Electronic Publication: 2018 Aug 08 (Print Publication: 2018).
DOI: 10.1371/journal.pone.0201969
Abstrakt: Candida albicans is one of the most common causes of hospital-acquired urinary tract infections (UTIs). However, azoles are poorly active against biofilms, echinocandins do not achieve clinically useful urinary concentrations, and amphotericin B exhibits severe toxicities. Thus, novel strategies are needed to prevent Candida UTIs, which are often associated with urinary catheter biofilms. We previously demonstrated that cranberry-derived proanthocyanidins (PACs) prevent C. albicans biofilm formation in an in vitro urinary model. To elucidate functional pathways unique to urinary biofilm development and PAC inhibition, we investigated the transcriptome of C. albicans in artificial urine (AU), with and without PACs. C. albicans biofilm and planktonic cells were cultivated with or without PACs. Genome-wide expression analysis was performed by RNA sequencing. Differentially expressed genes were determined using DESeq2 software; pathway analysis was performed using Cytoscape. Approximately 2,341 of 6,444 total genes were significantly expressed in biofilm relative to planktonic cells. Functional pathway analysis revealed that genes involved in filamentation, adhesion, drug response and transport were up-regulated in urinary biofilms. Genes involved in carbon and nitrogen metabolism and nutrient response were down-regulated. In PAC-treated urinary biofilms compared to untreated control biofilms, 557 of 6,444 genes had significant changes in gene expression. Genes downregulated in PAC-treated biofilms were implicated in iron starvation and adhesion pathways. Although urinary biofilms share key features with biofilms formed in other environments, many genes are uniquely expressed in urinary biofilms. Cranberry-derived PACs interfere with the expression of iron acquisition and adhesion genes within urinary biofilms.
Competing Interests: The authors have declared that no competing interests exist.
Databáze: MEDLINE
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