Proteomic and evolutionary analyses of sperm activation identify uncharacterized genes in Caenorhabditis nematodes.

Autor: Kasimatis KR; Institute of Ecology and Evolution, University of Oregon, 5289 University of Oregon, Eugene, OR, 97403-5289, USA., Moerdyk-Schauwecker MJ; Institute of Ecology and Evolution, University of Oregon, 5289 University of Oregon, Eugene, OR, 97403-5289, USA., Timmermeyer N; Institute of Ecology and Evolution, University of Oregon, 5289 University of Oregon, Eugene, OR, 97403-5289, USA., Phillips PC; Institute of Ecology and Evolution, University of Oregon, 5289 University of Oregon, Eugene, OR, 97403-5289, USA. pphil@uoregon.edu.
Jazyk: angličtina
Zdroj: BMC genomics [BMC Genomics] 2018 Aug 07; Vol. 19 (1), pp. 593. Date of Electronic Publication: 2018 Aug 07.
DOI: 10.1186/s12864-018-4980-7
Abstrakt: Background: Nematode sperm have unique and highly diverged morphology and molecular biology. In particular, nematode sperm contain subcellular vesicles known as membranous organelles that are necessary for male fertility, yet play a still unknown role in overall sperm function. Here we take a novel proteomic approach to characterize the functional protein complement of membranous organelles in two Caenorhabditis species: C. elegans and C. remanei.
Results: We identify distinct protein compositions between membranous organelles and the activated sperm body. Two particularly interesting and undescribed gene families-the Nematode-Specific Peptide family, group D and the here designated Nematode-Specific Peptide family, group F-localize to the membranous organelle. Both multigene families are nematode-specific and exhibit patterns of conserved evolution specific to the Caenorhabditis clade. These data suggest gene family dynamics may be a more prevalent mode of evolution than sequence divergence within sperm. Using a CRISPR-based knock-out of the NSPF gene family, we find no evidence of a male fertility effect of these genes, despite their high protein abundance within the membranous organelles.
Conclusions: Our study identifies key components of this unique subcellular sperm component and establishes a path toward revealing their underlying role in reproduction.
Databáze: MEDLINE
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