| Autor: |
Hfaiedh M; a Research Unit of Valorization of Active Biomolecules, Higher Institute of Applied Biology of Medenine, University of Gabes, 4119 Medenine, Tunisia., Brahmi D; a Research Unit of Valorization of Active Biomolecules, Higher Institute of Applied Biology of Medenine, University of Gabes, 4119 Medenine, Tunisia.; b Laboratory of Research on Biologically Compatible Substances, Faculty of Dental Medicine, University of Monastir, Rue Avicenne, 5019 Monastir, Tunisia., Zourgui MN; a Research Unit of Valorization of Active Biomolecules, Higher Institute of Applied Biology of Medenine, University of Gabes, 4119 Medenine, Tunisia.; c Higher Institute of Applied Biology of Medenine, University of Gabes, 4119 Medenine, Tunisia., Zourgui L; a Research Unit of Valorization of Active Biomolecules, Higher Institute of Applied Biology of Medenine, University of Gabes, 4119 Medenine, Tunisia.; c Higher Institute of Applied Biology of Medenine, University of Gabes, 4119 Medenine, Tunisia. |
| Jazyk: |
angličtina |
| Zdroj: |
Applied physiology, nutrition, and metabolism = Physiologie appliquee, nutrition et metabolisme [Appl Physiol Nutr Metab] 2019 Mar; Vol. 44 (3), pp. 239-247. Date of Electronic Publication: 2018 Aug 07. |
| DOI: |
10.1139/apnm-2018-0184 |
| Abstrakt: |
Environmental and occupational exposure to chromium compounds, especially hexavalent chromium, is widely recognized as potentially nephrotoxic in humans and animals. The present study aimed to assess the efficacy of cactus (Opuntia ficus-indica) against sodium dichromate-induced nephrotoxicity, oxidative stress, and genotoxicity. Cactus cladodes extract (CCE) was phytochemically studied and tested in vitro for its potential antioxidant activities. Additionally, the preventive effect of CCE against sodium dichromate-induced renal dysfunction in a Wistar rat model (24 rats) was evaluated. For this purpose, CCE at a dose of 100 mg/kg was orally administered, followed by 10 mg/kg sodium dichromate (intraperitoneal injection). After 40 days of treatment, the rats were sacrificed, and the kidneys were excised for histological, lipid peroxidation, and antioxidant enzyme analyses. The phenol, flavonoid, tannin, ascorbic acid, and carotenoid contents of CCE were considered to be important. Our analyses showed that 1 mL of CCE was equivalent to 982.5 ± 1.79 μg of gallic acid, 294.37 ± 0.84 μg of rutin, 234.78 ± 0.24 μg of catechin, 204.34 ± 1.53 μg of ascorbic acid, and 3.14 ± 0.51 μg of β-carotene. In vivo, pretreatment with CCE was found to provide significant protection against sodium dichromate-induced nephrotoxicity by inhibiting lipid peroxidation, preserving normal antioxidant activities, and protecting renal tissues from lesions and DNA damage. The nephroprotective potential of CCE against sodium dichromate toxicity might be due to its antioxidant properties. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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