Parallel Accumulation of Tumor Hyaluronan, Collagen, and Other Drivers of Tumor Progression.

Autor:	Li X; Halozyme Therapeutics, Inc., San Diego, California., Shepard HM; Halozyme Therapeutics, Inc., San Diego, California., Cowell JA; Halozyme Therapeutics, Inc., San Diego, California., Zhao C; Halozyme Therapeutics, Inc., San Diego, California., Osgood RJ; Halozyme Therapeutics, Inc., San Diego, California., Rosengren S; Halozyme Therapeutics, Inc., San Diego, California., Blouw B; Halozyme Therapeutics, Inc., San Diego, California., Garrovillo SA; Halozyme Therapeutics, Inc., San Diego, California., Pagel MD; Department of Cancer Systems Imaging, University of Texas, MD Anderson Cancer Center, Houston, Texas., Whatcott CJ; Clinical Translational Research Division, Translational Genomics Research Institute (TGen), Phoenix, Arizona., Han H; Clinical Translational Research Division, Translational Genomics Research Institute (TGen), Phoenix, Arizona., Von Hoff DD; Clinical Translational Research Division, Translational Genomics Research Institute (TGen), Phoenix, Arizona., Taverna DM; Halozyme Therapeutics, Inc., San Diego, California., LaBarre MJ; Halozyme Therapeutics, Inc., San Diego, California., Maneval DC; Halozyme Therapeutics, Inc., San Diego, California., Thompson CB; Halozyme Therapeutics, Inc., San Diego, California. cthompson@halozyme.com.
Jazyk:	angličtina
Zdroj:	Clinical cancer research : an official journal of the American Association for Cancer Research [Clin Cancer Res] 2018 Oct 01; Vol. 24 (19), pp. 4798-4807. Date of Electronic Publication: 2018 Jul 03.
DOI:	10.1158/1078-0432.CCR-17-3284
Abstrakt:	Purpose: The tumor microenvironment (TME) evolves to support tumor progression. One marker of more aggressive malignancy is hyaluronan (HA) accumulation. Here, we characterize biological and physical changes associated with HA-accumulating (HA-high) tumors. Experimental Design: We used immunohistochemistry, in vivo imaging of tumor pH, and microdialysis to characterize the TME of HA-high tumors, including tumor vascular structure, hypoxia, tumor perfusion by doxorubicin, pH, content of collagen. and smooth muscle actin (α-SMA). A novel method was developed to measure real-time tumor-associated soluble cytokines and growth factors. We also evaluated biopsies of murine and pancreatic cancer patients to investigate HA and collagen content, important contributors to drug resistance. Results: In immunodeficient and immunocompetent mice, increasing tumor HA content is accompanied by increasing collagen content, vascular collapse, hypoxia, and increased metastatic potential, as reflected by increased α-SMA. In vivo treatment of HA-high tumors with PEGylated recombinant human hyaluronidase (PEGPH20) dramatically reversed these changes and depleted stores of VEGF-A165, suggesting that PEGPH20 may also diminish the angiogenic potential of the TME. Finally, we observed in xenografts and in pancreatic cancer patients a coordinated increase in HA and collagen tumor content. Conclusions: The accumulation of HA in tumors is associated with high tIP, vascular collapse, hypoxia, and drug resistance. These findings may partially explain why more aggressive malignancy is observed in the HA-high phenotype. We have shown that degradation of HA by PEGPH20 partially reverses this phenotype and leads to depletion of tumor-associated VEGF-A165. These results encourage further clinical investigation of PEGPH20. Clin Cancer Res; 24(19); 4798-807. ©2018 AACR . (©2018 American Association for Cancer Research.)
Databáze:	MEDLINE
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