Gating of miRNA movement at defined cell-cell interfaces governs their impact as positional signals.

Autor:	Skopelitis DS; Cold Spring Harbor Laboratory, One Bungtown Road, Cold Spring Harbor, NY, 11724, USA., Hill K; Center for Plant Molecular Biology (ZMBP), University of Tübingen, Auf der Morgenstelle 32, 72076, Tübingen, Germany., Klesen S; Center for Plant Molecular Biology (ZMBP), University of Tübingen, Auf der Morgenstelle 32, 72076, Tübingen, Germany., Marco CF; Cold Spring Harbor Laboratory, One Bungtown Road, Cold Spring Harbor, NY, 11724, USA., von Born P; Cold Spring Harbor Laboratory, One Bungtown Road, Cold Spring Harbor, NY, 11724, USA., Chitwood DH; Cold Spring Harbor Laboratory, One Bungtown Road, Cold Spring Harbor, NY, 11724, USA.; Department Horticulture and Computational Mathematics, Science & Engineering, Michigan State University, 1066 Bogue Street, East Lansing, MI, 48824, USA., Timmermans MCP; Cold Spring Harbor Laboratory, One Bungtown Road, Cold Spring Harbor, NY, 11724, USA. marja.timmermans@zmbp.uni-tuebingen.de.; Center for Plant Molecular Biology (ZMBP), University of Tübingen, Auf der Morgenstelle 32, 72076, Tübingen, Germany. marja.timmermans@zmbp.uni-tuebingen.de.
Jazyk:	angličtina
Zdroj:	Nature communications [Nat Commun] 2018 Aug 06; Vol. 9 (1), pp. 3107. Date of Electronic Publication: 2018 Aug 06.
DOI:	10.1038/s41467-018-05571-0
Abstrakt:	Mobile small RNAs serve as local positional signals in development and coordinate stress responses across the plant. Despite its central importance, an understanding of how the cell-to-cell movement of small RNAs is governed is lacking. Here, we show that miRNA mobility is precisely regulated through a gating mechanism polarised at defined cell-cell interfaces. This generates directional movement between neighbouring cells that limits long-distance shoot-to-root trafficking, and underpins domain-autonomous behaviours of small RNAs within stem cell niches. We further show that the gating of miRNA mobility occurs independent of mechanisms controlling protein movement, identifying the small RNA as the mobile unit. These findings reveal gate-keepers of cell-to-cell small RNA mobility generate selectivity in long-distance signalling, and help safeguard functional domains within dynamic stem cell niches while mitigating a 'signalling gridlock' in contexts where developmental patterning events occur in close spatial and temporal vicinity.
Databáze:	MEDLINE
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