Promising anticancer activity of batumin: a natural polyene antibiotic produced by Pseudomonas batumici.

Autor: Soldatkina MA; Department of Molecular & Cellular Pathobiology, R.E. Kavetsky Institute of Experimental Pathology, Oncology & Radiobiology, NAS of Ukraine, Kyiv 03022, Ukraine., Klochko VV; Department of Antibiotics, DK Zabolotny Institute of Microbiology & Virology, National Academy of Science of Ukraine, Kyiv 03680, Ukraine., Zagorodnya SD; Department of Viruses Reproduction, DK Zabolotny Institute of Microbiology & Virology, National Academy of Science of Ukraine, Kyiv 03680, Ukraine., Rademan S; Department of Plant & Soil Sciences, Medicinal Plant Science Section, University of Pretoria, Pretoria 0002, South Africa., Visagie MH; Department of Physiology, University of Pretoria, Pretoria 0001, South Africa., Lebelo MT; Department of Physiology, University of Pretoria, Pretoria 0001, South Africa., Gwangwa MV; Department of Physiology, University of Pretoria, Pretoria 0001, South Africa., Joubert AM; Department of Physiology, University of Pretoria, Pretoria 0001, South Africa., Lall N; Department of Plant & Soil Sciences, Medicinal Plant Science Section, University of Pretoria, Pretoria 0002, South Africa., Reva ON; Department of Biochemistry, Genetics & Microbiology, Centre for Bioinformatics & Computational Biology, University of Pretoria, Pretoria 0002, South Africa.
Jazyk: angličtina
Zdroj: Future medicinal chemistry [Future Med Chem] 2018 Sep 01; Vol. 10 (18), pp. 2187-2199. Date of Electronic Publication: 2018 Aug 07.
DOI: 10.4155/fmc-2018-0062
Abstrakt: Aim: To determine the computer-predicted anticancer activity of antibiotic batumin.
Materials & Methods: Cytotoxicity assays, cell morphology microscopy and cell cycle progression were studied in cancer and nontumorigenic cell lines. An in vivo experiment on Lewis lung carcinoma (3LL)-transplanted mice was conducted to evaluate potential antimetastatic.
Results & Conclusion: Cytotoxicity against melanoma and lung carcinoma cells (IC 50 ≈ 5 μg/ml) was detected. Hypercondensed chromatin and apoptotic body formation in batumin-treated cells suggested the induction of apoptosis supported also by an observed increase in the quantity of cells occupying the sub-G1 cell cycle phase. Twofold reduction in the number and volume of lung metastases in Lewis lung carcinoma (3LL)-bearing batumin-treated mice was demonstrated. Highly specific cytotoxicity of batumin against cancer cell lines potentiates further studies.
Databáze: MEDLINE
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