The Dynamics of Replication in Trypanosoma cruzi Parasites by Single-Molecule Analysis.

Autor: de Araujo CB; Laboratorio Especial de Ciclo celular, Instituto Butantan, Av Vital Brasil, 1500, São Paulo, 05503-900, Brazil.; Center of Toxins, Immune Response and Cell Signaling (CeTICS), Instituto Butantan, Av Vital Brasil, 1500, São Paulo, 05503-900, Brazil., Calderano SG; Center of Toxins, Immune Response and Cell Signaling (CeTICS), Instituto Butantan, Av Vital Brasil, 1500, São Paulo, 05503-900, Brazil.; Laboratório de Parasitologia, Instituto Butantan, Av Vital Brasil, 1500, São Paulo, 05503-900, Brazil., Elias MC; Laboratorio Especial de Ciclo celular, Instituto Butantan, Av Vital Brasil, 1500, São Paulo, 05503-900, Brazil.; Center of Toxins, Immune Response and Cell Signaling (CeTICS), Instituto Butantan, Av Vital Brasil, 1500, São Paulo, 05503-900, Brazil.
Jazyk: angličtina
Zdroj: The Journal of eukaryotic microbiology [J Eukaryot Microbiol] 2019 May; Vol. 66 (3), pp. 514-518. Date of Electronic Publication: 2018 Aug 25.
DOI: 10.1111/jeu.12676
Abstrakt: Here, we investigated the features of replication in Trypanosoma cruzi epimastigotes based on fork speed progression, which is influenced by distinct features such as DNA polymerase rate, susceptibility to DNA damage and repair, secondary structures, transcription and chromatin state. Although T. cruzi exhibits a mean fork speed (2.05 ± 0.10 kb/min) very similar to other trypanosomatids, we found that the majority of DNA molecules replicated more slowly, with a frequency distribution approximately 1 kb/min. This frequency distribution analysis provides more information about the replication profile of this organism.
(© 2018 International Society of Protistologists.)
Databáze: MEDLINE
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