Enhancing the Cancer Cell Growth Inhibitory Effects of Table Grape Anthocyanins.

Autor: Grimes KL; Dept. of Chemistry and Biochemistry, California State Univ., Bakersfield, Bakersfield, Calif., U.S.A., Stuart CM; Dept. of Chemistry and Biochemistry, California State Univ., Bakersfield, Bakersfield, Calif., U.S.A., McCarthy JJ; Dept. of Chemistry and Biochemistry, California State Univ., Bakersfield, Bakersfield, Calif., U.S.A., Kaur B; Dept. of Chemistry and Biochemistry, California State Univ., Bakersfield, Bakersfield, Calif., U.S.A., Cantu EJ; Dept. of Chemistry and Biochemistry, California State Univ., Bakersfield, Bakersfield, Calif., U.S.A., Forester SC; Dept. of Chemistry and Biochemistry, California State Univ., Bakersfield, Bakersfield, Calif., U.S.A.
Jazyk: angličtina
Zdroj: Journal of food science [J Food Sci] 2018 Sep; Vol. 83 (9), pp. 2369-2374. Date of Electronic Publication: 2018 Aug 02.
DOI: 10.1111/1750-3841.14294
Abstrakt: The risk for breast and colon cancer may be lowered in part by high intake of fruits and vegetables. Fruits such as grapes are abundant in bioactive compounds such as anthocyanins. The potential anticancer activity of anthocyanins may be limited by their metabolism in the gut and liver. One metabolic transformation is due to the enzyme catechol-O-methyltransferase (COMT), which methylates polyphenols such as anthocyanins. Entacapone is a clinically used inhibitor of COMT, and has been shown to modulate the methylation of food-derived polyphenols. In this study, we compared the effect of entacapone on the cell viability of colon (Caco-2 and HT-29) and breast (MDA-MB-231) cancer cell lines treated with anthocyanins. Cells were treated with either cyanidin-3-glucoside, delphinidin-3-glucoside, or an anthocyanin-rich grape extract, in the absence or presence of entacapone. Cell viability was assessed using the thiazolyl blue tetrazolium bromide (MTT) assay. Entacapone in combination with the anthocyanins had a greater than additive effect on growth inhibition of the Caco-2 cells. In the MDA-MB-231 cell line, entacapone similarly enhanced the growth inhibitory activity of the anthocyanin extract. Entacapone also had antiproliferative effects when used as a single treatment. Total hydroperoxides was quantified in the cell culture media. Greater concentrations of the treatments resulted in higher levels of total hydroperoxides, indicating that oxidative stress may be an important mechanism for growth inhibition. In conclusion, the antiproliferative activity of fruit-derived anthocyanins was improved in human cancer cell lines by the clinically used drug entacapone. The efficacy and mechanisms of entacapone/anthocyanin combinations should be carefully studied in vivo.
Practical Application: Chemical components of grapes are good for our health and have been shown to lower risk for certain cancers. Their beneficial health effects could also be enhanced by consuming other molecules that improve their bioavailability.
(© 2018 Institute of Food Technologists®.)
Databáze: MEDLINE
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