Minimum requirements for changing and maintaining endodermis cell identity in the Arabidopsis root.

Autor: Drapek C; Biology Department, Duke University, Durham, NC, USA.; Howard Hughes Medical Institute, Duke University, Durham, NC, USA., Sparks EE; Department of Plant and Soil Sciences, University of Delaware, Newark, DE, USA., Marhavy P; Department of Plant Molecular Biology, University of Lausanne, Lausanne, Switzerland., Taylor I; Biology Department, Duke University, Durham, NC, USA.; Howard Hughes Medical Institute, Duke University, Durham, NC, USA., Andersen TG; Department of Plant Molecular Biology, University of Lausanne, Lausanne, Switzerland., Hennacy JH; Biology Department, Duke University, Durham, NC, USA.; Princeton University, Princeton, NJ, USA., Geldner N; Department of Plant Molecular Biology, University of Lausanne, Lausanne, Switzerland., Benfey PN; Biology Department, Duke University, Durham, NC, USA. philip.benfey@duke.edu.; Howard Hughes Medical Institute, Duke University, Durham, NC, USA. philip.benfey@duke.edu.
Jazyk: angličtina
Zdroj: Nature plants [Nat Plants] 2018 Aug; Vol. 4 (8), pp. 586-595. Date of Electronic Publication: 2018 Jul 30.
DOI: 10.1038/s41477-018-0213-y
Abstrakt: Changes in gene regulation during differentiation are governed by networks of transcription factors. The Arabidopsis root endodermis is a tractable model to address how transcription factors contribute to differentiation. We used a bottom-up approach to understand the extent to which transcription factors that are required for endodermis differentiation can confer endodermis identity to a non-native cell type. Our results show that the transcription factors SHORTROOT and MYB36 alone have limited ability to induce ectopic endodermal features in the absence of additional cues. The stele-derived signalling peptide CIF2 stabilizes SHORTROOT-induced endodermis identity acquisition. The outcome is a partially impermeable barrier deposited in the subepidermal cell layer, which has a transcriptional signature similar to the endodermis. These results demonstrate that other root cell types can be forced to differentiate into the endodermis and highlight a previously unappreciated role for receptor kinase signalling in maintaining endodermis identity.
Databáze: MEDLINE
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