Labeling of Collagen Type I Templates with a Naturally Derived Contrast Agent for Noninvasive MR Imaging in Soft Tissue Engineering.

Autor: Janke HP; Department of Urology, Radboud Institute for Molecular Life Sciences, Radboud University Medical Center, Geert Grooteplein 28, 6525 GA, Nijmegen, The Netherlands., Güvener N; Institute for Experimental Molecular Imaging, Center for Biohybrid Medical Systems Uniklinik RWTH and Helmholtz Institute for Biomedical Engineering, RWTH Aachen University, Forckenbeckstr. 55, 52074, Aachen, Germany.; Nano4Imaging GmbH, Zentrum für Biomedizintechnik (ZBMT), Pauwelsstrasse 17, 52074, Aachen, Germany., Dou W; Department of Radiology and Nuclear Medicine, Radboud University Medical Center, PO Box 9101, 6500 HB, Nijmegen, The Netherlands.; GE Healthcare, MR Research China, Beijing, 100176, China., Tiemessen DM; Department of Urology, Radboud Institute for Molecular Life Sciences, Radboud University Medical Center, Geert Grooteplein 28, 6525 GA, Nijmegen, The Netherlands., YantiSetiasti A; Department of Urology, Radboud Institute for Molecular Life Sciences, Radboud University Medical Center, Geert Grooteplein 28, 6525 GA, Nijmegen, The Netherlands.; Department of Anatomical Pathology, Faculty of Medicine, University of Padjadjaran, Jalan Professor Eyckman No. 38, Bandung, 4016, Indonesia., Cremers JGO; Institute for Experimental Molecular Imaging, Center for Biohybrid Medical Systems Uniklinik RWTH and Helmholtz Institute for Biomedical Engineering, RWTH Aachen University, Forckenbeckstr. 55, 52074, Aachen, Germany.; Nano4Imaging GmbH, Zentrum für Biomedizintechnik (ZBMT), Pauwelsstrasse 17, 52074, Aachen, Germany., Borm PJA; Nano4Imaging GmbH, Zentrum für Biomedizintechnik (ZBMT), Pauwelsstrasse 17, 52074, Aachen, Germany., Feitz WFJ; Department of Urology, Radboud Institute for Molecular Life Sciences, Radboud University Medical Center, Geert Grooteplein 28, 6525 GA, Nijmegen, The Netherlands.; Radboudumc Amalia Children's Hospital, Radboud University Medical Center, Geert Grooteplein 28, 6525 GA, Nijmegen, The Netherlands., Heerschap A; Department of Radiology and Nuclear Medicine, Radboud University Medical Center, PO Box 9101, 6500 HB, Nijmegen, The Netherlands., Kiessling F; Institute for Experimental Molecular Imaging, Center for Biohybrid Medical Systems Uniklinik RWTH and Helmholtz Institute for Biomedical Engineering, RWTH Aachen University, Forckenbeckstr. 55, 52074, Aachen, Germany., Oosterwijk E; Department of Urology, Radboud Institute for Molecular Life Sciences, Radboud University Medical Center, Geert Grooteplein 28, 6525 GA, Nijmegen, The Netherlands.
Jazyk: angličtina
Zdroj: Advanced healthcare materials [Adv Healthc Mater] 2018 Sep; Vol. 7 (18), pp. e1800605. Date of Electronic Publication: 2018 Jul 29.
DOI: 10.1002/adhm.201800605
Abstrakt: In vivo monitoring of tissue-engineered constructs is important to assess their integrity, remodeling, and degradation. However, this is challenging when the contrast with neighboring tissues is low, necessitating labeling with contrast agents (CAs), but current CAs have limitations (i.e., toxicity, negative contrast, label instability, and/or inappropriate size). Therefore, a naturally derived hemin-L-lysine (HL) complex is used as a potential CA to label collagen-based templates for magnetic resonance imaging (MRI). Labeling does not change the basic characteristics of the collagen templates. When hybrid templates composed of collagen type I reinforced with degradable polymers are subcutaneously implanted in mice, longitudinal visualization by MRI is possible with good contrast and in correlation with template remodeling. In contrast, unlabeled collagen templates are hardly detectable and the fate of these templates cannot be monitored by MRI. Interestingly, tissue remodeling and vascularization are enhanced within HL-labeled templates. Thus, HL labeling is presented as a promising universal imaging marker to label tissue-engineered implants for MRI, which additionally seems to accelerate tissue regeneration.
(© 2018 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.)
Databáze: MEDLINE
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