| Autor: |
Chmielewska JJ; Laboratory of Molecular Basis of Synaptic Plasticity, Centre of New Technologies, University of Warsaw, S. Banacha 2c, 02-097, Warsaw, Poland.; Postgraduate School of Molecular Medicine, Medical University of Warsaw, Zwirki i Wigury 61, 02-091, Warsaw, Poland., Kuzniewska B; Laboratory of Molecular Basis of Synaptic Plasticity, Centre of New Technologies, University of Warsaw, S. Banacha 2c, 02-097, Warsaw, Poland., Milek J; Laboratory of Molecular Basis of Synaptic Plasticity, Centre of New Technologies, University of Warsaw, S. Banacha 2c, 02-097, Warsaw, Poland., Urbanska K; Laboratory of Molecular Basis of Synaptic Plasticity, Centre of New Technologies, University of Warsaw, S. Banacha 2c, 02-097, Warsaw, Poland., Dziembowska M; Laboratory of Molecular Basis of Synaptic Plasticity, Centre of New Technologies, University of Warsaw, S. Banacha 2c, 02-097, Warsaw, Poland. m.dziembowska@cent.uw.edu.pl. |
| Abstrakt: |
Neuroligins (NLGNs) are cell adhesion molecules located on the postsynaptic side of the synapse that interact with their presynaptic partners neurexins to maintain trans-synaptic connection. Fragile X syndrome (FXS) is a common neurodevelopmental disease that often co-occurs with autism and is caused by the lack of fragile X mental retardation protein (FMRP) expression. To gain an insight into the molecular interactions between the autism-related genes, we sought to determine whether FMRP controls the synaptic levels of NLGNs. We show evidences that FMRP associates with Nlgn1, Nlgn2, and Nlgn3 mRNAs in vitro in both synaptoneurosomes and neuronal cultures. Next, we confirm local translation of Nlgn1, Nlgn2, and Nlgn3 mRNAs to be synaptically regulated by FMRP. As a consequence of elevated Nlgns mRNA translation Fmr1 KO mice exhibit increased incorporation of NLGN1 and NLGN3 into the postsynaptic membrane. Finally, we show that neuroligins synaptic level is precisely and dynamically regulated by their rapid proteolytic cleavage upon NMDA receptor stimulation in both wild type and Fmr1 KO mice. In aggregate, our study provides a novel approach to understand the molecular basis of FXS by linking the dysregulated synaptic expression of NLGNs with FMRP. |
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