Providing direction improves function: Comparison of a radial pore-orientated acellular collagen scaffold to clinical alternatives in a surgically induced rabbit diaphragmatic tissue defect model.

Autor:	Eastwood MP; Department of Development and Regeneration, Katholieke Universiteit Leuven, Leuven, Belgium.; Center for Surgical Technologies, Katholieke Universiteit Leuven, Leuven, Belgium., Daamen WF; Department of Biochemistry, RIMLS, Radboud University Medical Center, Nijmegen, The Netherlands., Joyeux L; Department of Development and Regeneration, Katholieke Universiteit Leuven, Leuven, Belgium.; Center for Surgical Technologies, Katholieke Universiteit Leuven, Leuven, Belgium., Pranpanus S; Department of Development and Regeneration, Katholieke Universiteit Leuven, Leuven, Belgium.; Center for Surgical Technologies, Katholieke Universiteit Leuven, Leuven, Belgium.; Department of Obstetrics and Gynecology, Prince of Songkla University, Hat Yai, Thailand., Rynkevic R; Department of Development and Regeneration, Katholieke Universiteit Leuven, Leuven, Belgium.; NEGI, Faculdade de Engenharia da Universidade do Porto, Universidade do Porto, Porto, Portugal., Hympanova L; Department of Development and Regeneration, Katholieke Universiteit Leuven, Leuven, Belgium.; Center for Surgical Technologies, Katholieke Universiteit Leuven, Leuven, Belgium.; Institute for the Care of the Mother and Child, Third Faculty of Medicine, Charles University, Prague, Czech Republic., Pot MW; Department of Biochemistry, RIMLS, Radboud University Medical Center, Nijmegen, The Netherlands., Hof DJ; Department of Biochemistry, RIMLS, Radboud University Medical Center, Nijmegen, The Netherlands., Gayan-Ramirez G; Laboratory of Pneumology, Katholieke Universiteit Leuven, Leuven, Belgium., van Kuppevelt TH; Department of Biochemistry, RIMLS, Radboud University Medical Center, Nijmegen, The Netherlands., Verbeken E; Department of Pathology, Group Biomedical Sciences, University Hospitals Leuven, Leuven, Belgium., Deprest J; Department of Development and Regeneration, Katholieke Universiteit Leuven, Leuven, Belgium.; Center for Surgical Technologies, Katholieke Universiteit Leuven, Leuven, Belgium.; Research Department of Maternal Fetal Medicine, Institute of Women's Health, University College London, London, UK.
Jazyk:	angličtina
Zdroj:	Journal of tissue engineering and regenerative medicine [J Tissue Eng Regen Med] 2018 Nov; Vol. 12 (11), pp. 2138-2150. Date of Electronic Publication: 2018 Sep 12.
DOI:	10.1002/term.2734
Abstrakt:	Gore-Tex® is a widely used durable patch for repair of congenital diaphragmatic defects yet may result in complications. We compared Gore-Tex with a composite of a radial pore-orientated collagen scaffold (RP-Composite) and clinically used porcine small intestinal submucosa (SIS; Surgisis®) in a rabbit model for diaphragmatic hernia. The growing rabbit mimics the rapid rib cage growth and reherniation rates seen in children. We created and immediately repaired left hemidiaphragmatic defects in 6-week-old rabbits with Gore-Tex, SIS, and an RP-Composite scaffold. An additional group of rabbits had a sham operation. At 90 days, survivors more than doubled in weight. We observed few reherniations or eventrations in Gore-Tex (17%) and RP-Composite (22%) implanted animals. However, SIS failed in all rabbits. Maximum transdiaphragmatic pressure was lower in Gore-Tex (71%) than RP-Composite implanted animals (112%) or sham (134%). Gore-Tex repairs were less compliant than RP-Composite, which behaved as sham diaphragm (p < 0.01). RP-Composite induced less foreign body giant cell reaction than Gore-Tex (p < 0.05) with more collagen deposition (p < 0.001), although there was a tendency for the scaffold to calcify. Unlike Gore-Tex, the compliance of diaphragms reconstructed with RP-Composite scaffolds were comparable with native diaphragm, whereas reherniation rates and transdiaphragmatic pressure measurements were similar. (© 2018 John Wiley & Sons, Ltd.)
Databáze:	MEDLINE
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