Licochalcone A from licorice root, an inhibitor of human hepatoma cell growth via induction of cell apoptosis and cell cycle arrest.

Autor: Wang J; School of Food Science and Engineering, Hefei University of Technology, Hefei, 230009, People's Republic of China. Electronic address: hfutwangjun@163.com., Zhang YS; School of Food Science and Engineering, Hefei University of Technology, Hefei, 230009, People's Republic of China. Electronic address: ying_shuo_zhang@163.com., Thakur K; School of Food Science and Engineering, Hefei University of Technology, Hefei, 230009, People's Republic of China. Electronic address: kumarikiran@hfut.edu.cn., Hussain SS; School of Food Science and Engineering, Hefei University of Technology, Hefei, 230009, People's Republic of China. Electronic address: sajid_biotech1214@yahoo.com., Zhang JG; School of Food Science and Engineering, Hefei University of Technology, Hefei, 230009, People's Republic of China; Anhui Province Key Laboratory of Functional Compound Seasoning, Anhui Qiangwang Seasoning Food Co., Ltd, Jieshou, 236500, People's Republic of China. Electronic address: zhangjianguo@hfut.edu.cn., Xiao GR; School of Food Science and Engineering, Hefei University of Technology, Hefei, 230009, People's Republic of China. Electronic address: xiaoguiran.101@163.com., Wei ZJ; School of Food Science and Engineering, Hefei University of Technology, Hefei, 230009, People's Republic of China; Anhui Province Key Laboratory of Functional Compound Seasoning, Anhui Qiangwang Seasoning Food Co., Ltd, Jieshou, 236500, People's Republic of China. Electronic address: zjwei@hfut.edu.cn.
Jazyk: angličtina
Zdroj: Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association [Food Chem Toxicol] 2018 Oct; Vol. 120, pp. 407-417. Date of Electronic Publication: 2018 Jul 25.
DOI: 10.1016/j.fct.2018.07.044
Abstrakt: We investigated the anti-cancer activity of Licochalcone A (LCA), extracted from licorice root. LCA inhibited the proliferation of HepG 2 cells with IC 50 (65.96 μM) for 24 h and IC 50 (44.13 μM) for 48 h and caused significant morphological changes and also led to intracellular ROS generation. LCA affected HepG 2 cell growth by terminating cell cycle development at G2/M transition and further induced the apoptosis process. The mRNA expression of genes involved in cell cycles such as Survivin, Cyclin B1, and CDK1 were reduced; while, Weel, P21, Cyclin D1, and JNK1 showed increased mRNA expression. Two pathways consisting of internal and external factors were responsible for LCA -induced apoptosis. The anti-cancer action involved increased mRNA expression of DR3, DR5, caspases-3, caspases-8, caspases-10, Fas, Bad, Bax, Bcl-2, Bak, and PUMA; besides, decreased level of PKCε, p70S6K, and Akt. This study provides mechanistic explanation for anti-cancer activity of LCA and also suggests its potential role in the treatment of hepatoma cancer.
(Copyright © 2018 Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE