Decreased microbiome diversity in the HIV small airway epithelium.

Autor: Xu S; Centre for Heart Lung Innovation, University of British Columbia, Vancouver, BC, Canada., Tsai A; Department of Medicine, Faculty of Medicine, University of British Columbia, Vancouver, BC, Canada., Sze MA; Department of Microbiology and Immunology, University of Michigan, Ann Arbor, MI, USA., Vucic EA; British Columbia Cancer Agency, Vancouver, BC, Canada., Shaipanich T; Division of Respiratory Medicine, St. Paul's Hospital, Vancouver, BC, Canada., Harris M; British Columbia Centre for Excellence in HIV/AIDS, St. Paul's Hospital, Vancouver, BC, Canada., Guillemi S; British Columbia Centre for Excellence in HIV/AIDS, St. Paul's Hospital, Vancouver, BC, Canada., Yang J; Centre for Heart Lung Innovation, University of British Columbia, Vancouver, BC, Canada., Sinha S; Faculty of Pharmaceutical Sciences, University of British Columbia, Vancouver, BC, Canada., Nislow C; Faculty of Pharmaceutical Sciences, University of British Columbia, Vancouver, BC, Canada., Montaner J; British Columbia Centre for Excellence in HIV/AIDS, St. Paul's Hospital, Vancouver, BC, Canada., Lam W; British Columbia Cancer Agency, Vancouver, BC, Canada., Lam S; British Columbia Cancer Agency, Vancouver, BC, Canada., Sin DD; Centre for Heart Lung Innovation, University of British Columbia, Vancouver, BC, Canada.; Division of Respiratory Medicine, St. Paul's Hospital, Vancouver, BC, Canada., Paul Man SF; Centre for Heart Lung Innovation, University of British Columbia, Vancouver, BC, Canada.; Division of Respiratory Medicine, St. Paul's Hospital, Vancouver, BC, Canada., Leung JM; Centre for Heart Lung Innovation, University of British Columbia, Vancouver, BC, Canada. Janice.Leung@hli.ubc.ca.; Division of Respiratory Medicine, St. Paul's Hospital, Vancouver, BC, Canada. Janice.Leung@hli.ubc.ca.; Centre for Heart Lung Innovation, St. Paul's Hospital, Room 166-1081 Burrard Street, Vancouver, V6Z 1Y6, Canada. Janice.Leung@hli.ubc.ca.
Jazyk: angličtina
Zdroj: Respiratory research [Respir Res] 2018 Jul 27; Vol. 19 (1), pp. 140. Date of Electronic Publication: 2018 Jul 27.
DOI: 10.1186/s12931-018-0835-7
Abstrakt: Background: Persons living with human immunodeficiency virus (PLWH) face an increased burden of chronic obstructive pulmonary disease (COPD). Repeated pulmonary infections, antibiotic exposures, and immunosuppression may contribute to an altered small airway epithelium (SAE) microbiome.
Methods: SAE cells were collected from 28 PLWH and 48 HIV- controls through bronchoscopic cytologic brushings. DNA extracted from SAE cells was subjected to 16S rRNA amplification and sequencing. Comparisons of alpha and beta diversity between HIV+ and HIV- groups were performed and key operational taxonomic units (OTUs) distinguishing the two groups were identified using the Boruta feature selection after Random Forest Analysis.
Results: PLWH demonstrated significantly reduced Shannon diversity compared with HIV- volunteers (1.82 ± 0.10 vs. 2.20 ± 0.073, p = 0.0024). This was primarily driven by a reduction in bacterial richness (23.29 ± 2.75 for PLWH and 46.04 ± 3.716 for HIV-, p < 0.0001). Phyla distribution was significantly altered among PLWH, with an increase in relative abundance of Proteobacteria (p = 0.0003) and a decrease in Bacteroidetes (p = 0.0068) and Firmicutes (p = 0.0002). Six discriminative OTUs were found to distinguish PLWH from HIV- volunteers, aligning to Veillonellaceae, Fusobacterium, Verrucomicrobiaceae, Prevotella, Veillonella, and Campylobacter.
Conclusions: Compared to HIV- controls, PLWH's SAE microbiome is marked by reduced bacterial diversity and richness with significant differences in community composition.
Databáze: MEDLINE
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