Non-Enzymatic Tissue Homogenization for Biodistribution Analysis.

Autor:	DiPerna DM; Barrow Brain Tumor Research Center, Barrow Neurological Institute, St. Joseph's Hospital and Medical Center, Phoenix, AZ, USA., Prakapenka AV; Barrow Brain Tumor Research Center, Barrow Neurological Institute, St. Joseph's Hospital and Medical Center, Phoenix, AZ, USA.; Department of Psychology, Arizona State University, Tempe, AZ, USA., Chung EP; Barrow Brain Tumor Research Center, Barrow Neurological Institute, St. Joseph's Hospital and Medical Center, Phoenix, AZ, USA., Sirianni RW; Vivian L. Smith Department of Neurosurgery, University of Texas Health Science Center at Houston, Houston, TX, USA. rachael.sirianni@dignityhealth.org.; Barrow Neurological Institute, Phoenix, AZ, USA. rachael.sirianni@dignityhealth.org.
Jazyk:	angličtina
Zdroj:	Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2018; Vol. 1831, pp. 191-199.
DOI:	10.1007/978-1-4939-8661-3_14
Abstrakt:	Biodistribution is a valuable technique used to determine payload delivery from nanocarrier to organs of interest in preclinical models. Fluorescent probes can be used as drug surrogates, providing indirect but relevant measurement of tissue exposure to the carrier. This may be useful, for example, to perform a first-pass evaluation of how targeting affects delivery of encapsulated compounds to target organs. This protocol is designed for non-enzymatic tissue homogenization of a variety of organ types allowing tracking of small molecule fluorophores injected freely or encapsulated in nanoparticles.
Databáze:	MEDLINE
Externí odkaz:	Zobrazit plný text záznamu