Xylitol production from lignocellulosic whole slurry corn cob by engineered industrial Saccharomyces cerevisiae PE-2.

Autor: Baptista SL; CEB - Centre of Biological Engineering, University of Minho, 4710-057 Braga, Portugal., Cunha JT; CEB - Centre of Biological Engineering, University of Minho, 4710-057 Braga, Portugal., Romaní A; CEB - Centre of Biological Engineering, University of Minho, 4710-057 Braga, Portugal., Domingues L; CEB - Centre of Biological Engineering, University of Minho, 4710-057 Braga, Portugal. Electronic address: luciliad@deb.uminho.pt.
Jazyk: angličtina
Zdroj: Bioresource technology [Bioresour Technol] 2018 Nov; Vol. 267, pp. 481-491. Date of Electronic Publication: 2018 Jul 21.
DOI: 10.1016/j.biortech.2018.07.068
Abstrakt: In this work, the industrial Saccharomyces cerevisiae PE-2 strain, presenting innate capacity for xylitol accumulation, was engineered for xylitol production by overexpression of the endogenous GRE3 gene and expression of different xylose reductases from Pichia stipitis. The best-performing GRE3-overexpressing strain was capable to produce 148.5 g/L of xylitol from high xylose-containing media, with a 0.95 g/g yield, and maintained close to maximum theoretical yields (0.89 g/g) when tested in non-detoxified corn cob hydrolysates. Furthermore, a successful integrated strategy was developed for the production of xylitol from whole slurry corn cob in a presaccharification and simultaneous saccharification and fermentation process (15% solid loading and 36 FPU) reaching xylitol yield of 0.93 g/g and a productivity of 0.54 g/L·h. This novel approach results in an intensified valorization of lignocellulosic biomass for xylitol production in a fully integrated process and represents an advance towards a circular economy.
(Copyright © 2018 Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
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