Location and functions of Inebriated in the Drosophila eye.
| Autor: | Borycz J; Department of Psychology and Neuroscience, Dalhousie University, Halifax, NS B3H 4R2, Canada., Ziegler A; Receptor Biochemistry, Faculty of Chemistry and Biochemistry, Ruhr University of Bochum, 44780 Bochum, Germany., Borycz JA; Department of Psychology and Neuroscience, Dalhousie University, Halifax, NS B3H 4R2, Canada., Uhlenbrock G; Receptor Biochemistry, Faculty of Chemistry and Biochemistry, Ruhr University of Bochum, 44780 Bochum, Germany., Tapken D; Receptor Biochemistry, Faculty of Chemistry and Biochemistry, Ruhr University of Bochum, 44780 Bochum, Germany., Caceres L; Department of Psychology and Neuroscience, Dalhousie University, Halifax, NS B3H 4R2, Canada., Hollmann M; Molecular Cell Biochemistry, Ruhr University of Bochum, 44780 Bochum, Germany., Hovemann BT; Receptor Biochemistry, Faculty of Chemistry and Biochemistry, Ruhr University of Bochum, 44780 Bochum, Germany., Meinertzhagen IA; Department of Psychology and Neuroscience, Dalhousie University, Halifax, NS B3H 4R2, Canada jborycz@dal.ca.; Department of Biology, Dalhousie University, Halifax, NS B3H 4R2, Canada. |
|---|---|
| Jazyk: | angličtina |
| Zdroj: | Biology open [Biol Open] 2018 Jul 23; Vol. 7 (7). Date of Electronic Publication: 2018 Jul 23. |
| DOI: | 10.1242/bio.034926 |
| Abstrakt: | Histamine (HA) is a neurotransmitter in arthropod photoreceptors. It is recycled via conjugation to β-alanine to form β-alanylhistamine (carcinine). Conjugation occurs in epithelial glia that surround photoreceptor terminals in the first optic neuropil, and carcinine (CA) is then transported back to photoreceptors and cleaved to liberate HA and β-alanine. The gene Inebriated ( Ine ) encodes an Na + /Cl - -dependent SLC6 family transporter translated as two protein isoforms, long (P1) and short (P2). Photoreceptors specifically express Ine-P2 whereas Ine-P1 is expressed in non-neuronal cells. Both ine 1 and ine 3 have significantly reduced head HA contents compared with wild type, and a smaller increase in head HA after drinking 1% CA. Similarly, uptake of 0.1% CA was reduced in ine 1 and ine 3 mutant synaptosomes, but increased by 90% and 84% respectively for fractions incubated in 0.05% β-Ala, compared with wild type. Screening potential substrates in Ine expressing Xenopus oocytes revealed very little response to carcinine and β-Ala but increased conductance with glycine. Both ine 1 and ine 3 mutant responses in light-dark phototaxis did not differ from wild-type. Collectively our results suggest that Inebriated functions in an adjunct role as a transporter to the previously reported carcinine transporter CarT. Competing Interests: Competing interestsThe authors declare no competing or financial interests. (© 2018. Published by The Company of Biologists Ltd.) |
| Databáze: | MEDLINE |
| Externí odkaz: |
|