Comparison of the Purelyse® - IS6110 nested PCR with the Xpert® MTB/RIF test in clinical samples with suspected tuberculosis.

Autor: Lemus-Minor CG; Laboratorio Estatal de Salud Pública de Baja California, Instituto de Servicios de Salud Pública del Estado de B.C. (ISESALUD), Mexico; Laboratorio de Patogénesis Molecular, Facultad de Medicina Mexicali, Universidad Autónoma de Baja California, Mexico., Ovalle-Marroqui DF; Dirección de Enseñanza y Vinculación, Instituto de Servicios de Salud Pública del Estado de B.C. (ISESALUD), Mexico., Vazquez-Jimenez JG; Laboratorio de Patogénesis Molecular, Facultad de Medicina Mexicali, Universidad Autónoma de Baja California, Mexico., Reales-Agüero DL; Laboratorio de Patogénesis Molecular, Facultad de Medicina Mexicali, Universidad Autónoma de Baja California, Mexico., Sepulveda-Alcantara PM; Laboratorio de Patogénesis Molecular, Facultad de Medicina Mexicali, Universidad Autónoma de Baja California, Mexico., Rodriguez-Sánchez JR; Laboratorio de Patogénesis Molecular, Facultad de Medicina Mexicali, Universidad Autónoma de Baja California, Mexico., Diaz-Molina R; Laboratorio de Bioquímica, Facultad de Medicina Mexicali, Universidad Autónoma de Baja California, Mexico., Machado-Contreras JR; Laboratorio de Patogénesis Molecular, Facultad de Medicina Mexicali, Universidad Autónoma de Baja California, Mexico. Electronic address: rene.machado@uabc.edu.mx.
Jazyk: angličtina
Zdroj: Journal of microbiological methods [J Microbiol Methods] 2018 Sep; Vol. 152, pp. 48-51. Date of Electronic Publication: 2018 Jul 20.
DOI: 10.1016/j.mimet.2018.07.002
Abstrakt: Tuberculosis (TB) has a high incidence, prevalence and mortality in the world. Due to its high level of transmission and long-term pharmacological treatment, it is important to have sensitive and specific diagnostic tests. Recently, the PureLyse® system, which is a novel DNA extraction method, was proposed to be an important tool for molecular diagnosis of TB. Here, we compare the PureLyse® system followed by an IS6110 nested PCR (PureLyse® - IS6110 nested PCR) with the Xpert® MTB/RIF test for Mycobacterium tuberculosis complex (MTBC) identification in 40 clinical samples. Among the 40 samples, 26 samples were positive and 14 negative for the Xpert® MTB/RIF test as well as for the PureLyse® - IS6110 nested PCR. According to the Xpert® MTB/RIF test, positive samples presented different bacillary concentrations from "High" to "Very low" and rifampin resistance was observed in 5 samples. The concordance of both molecular methods makes the PureLyse® - IS6110 nested PCR suitable for MTBC detection in patients for low-income resources.
(Copyright © 2018 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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