Role of hypothetical protein YicS in the pathogenicity of Avian Pathogenic Escherichia coli in vivo and in vitro.

Autor: Verma R; Department of Genetics, Evolution and Bioagents, Institute of Biology, PO Box 6109, State University of Campinas-UNICAMP, 13083-875, Campinas, SP, Brazil. Electronic address: renuverma78@yahoo.co.in., Rojas TCG; Department of Genetics, Evolution and Bioagents, Institute of Biology, PO Box 6109, State University of Campinas-UNICAMP, 13083-875, Campinas, SP, Brazil., Maluta RP; Department of Genetics, Evolution and Bioagents, Institute of Biology, PO Box 6109, State University of Campinas-UNICAMP, 13083-875, Campinas, SP, Brazil., Leite JL; Department of Genetics, Evolution and Bioagents, Institute of Biology, PO Box 6109, State University of Campinas-UNICAMP, 13083-875, Campinas, SP, Brazil., Nakazato G; Department of Microbiology, Biological Science Center, Institute of Biology, University of Londrina-UEL, 86055-990, Londrina, PR, Brazil., de Silveira WD; Department of Genetics, Evolution and Bioagents, Institute of Biology, PO Box 6109, State University of Campinas-UNICAMP, 13083-875, Campinas, SP, Brazil. Electronic address: wds@unicamp.br.
Jazyk: angličtina
Zdroj: Microbiological research [Microbiol Res] 2018 Sep; Vol. 214, pp. 28-36. Date of Electronic Publication: 2018 May 13.
DOI: 10.1016/j.micres.2018.05.009
Abstrakt: Avian Pathogenic Escherichia coli (APEC) strains belong to the extra-intestinal pathogenic group of E. coli (ExPEC) that causes colibacillosis in poultry. A variety of putative virulence factors of APEC are recognized as potent causes of pathogenicity, the mechanisms underlying their pathogenicity are still not fully understood. The role of yicS in the virulence of pathogenic E. coli is still unclear. Thus, yicS may be related to biofilm formation, which in some bacteria plays a role in pathogenicity. Therefore, the fact that this gene appears to be under positive selection pressure suggests that yicS may be associated with the pathogenicity of APEC. To better understand the role of yicS protein in APEC biological characteristics and pathogenicity, we deleted yicS in an APEC Swollen Head Syndrome strain (APEC strain SCI-07) and studied its effects by comparing wild type and isogenic mutants through comprehensive in vitro and in vivo assays. We demonstrated that yicS plays a role in pathogenicity of APEC. We suggest that the yicS gene, which encodes an exporter protein, has a significant role in biofilm formation, motility, invasion of CEC-32 and Hep-2 cells and APEC pathogenicity in a day-old chick model.
(Copyright © 2018 Elsevier GmbH. All rights reserved.)
Databáze: MEDLINE
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