Proteomic and transcriptomic approaches to identify resistance and susceptibility related proteins in contrasting rice genotypes infected with fungal pathogen Rhizoctonia solani.
Autor: | Prathi NB; ICAR-Indian Institute of Rice Research (IIRR), Hyderabad, 500030, India., Palit P; ICAR-Indian Institute of Rice Research (IIRR), Hyderabad, 500030, India; International Crops Research Institute for the Semi-Arid Tropics (ICRISAT), Patancheru, 502324, India., Madhu P; ICAR-Indian Institute of Rice Research (IIRR), Hyderabad, 500030, India., M R; ICAR-Indian Institute of Rice Research (IIRR), Hyderabad, 500030, India., Laha GS; ICAR-Indian Institute of Rice Research (IIRR), Hyderabad, 500030, India., Balachandran SM; ICAR-Indian Institute of Rice Research (IIRR), Hyderabad, 500030, India., Madhav MS; ICAR-Indian Institute of Rice Research (IIRR), Hyderabad, 500030, India., Sundaram RM; ICAR-Indian Institute of Rice Research (IIRR), Hyderabad, 500030, India., Mangrauthia SK; ICAR-Indian Institute of Rice Research (IIRR), Hyderabad, 500030, India. Electronic address: skmdrr@gmail.com. |
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Jazyk: | angličtina |
Zdroj: | Plant physiology and biochemistry : PPB [Plant Physiol Biochem] 2018 Sep; Vol. 130, pp. 258-266. Date of Electronic Publication: 2018 Jul 12. |
DOI: | 10.1016/j.plaphy.2018.07.012 |
Abstrakt: | The devastating sheath blight disease caused by Rhizoctonia solani Kuhn (teleomorph: Thanatephorus cucumeris) causes major yield loss in most rice growing regions of the world. In this study, two moderately tolerant and four susceptible genotypes of rice were selected for R. solani induced proteome analysis using two-dimensional polyacrylamide gel electrophoresis. Forty five differentially expressed proteins (DEPs) were identified and analyzed by Mass Spectrometry. Based on their functions, these proteins were classified into different groups, viz., photosynthesis, resistance and pathogenesis, stress, cell wall metabolism and cytoskeleton development associated proteins, and hypothetical or uncharacterized proteins. Expression of 14 genes encoding DEPs was analyzed by quantitative PCR which showed consistency in transcripts and genes expression pattern. Furthermore, the expression of 16 other genes involved in diverse biological functions was analyzed. Up-regulation of these genes in the tolerant genotype Pankaj during sheath blight disease suggested efficient genetic regulation of this cultivar under stress. Also, expression analysis of conserved microRNAs (miRNAs) and their target genes revealed important role of miRNAs in post-transcriptional gene regulation during development of rice sheath blight disease. Genome-wide discovery of miRNAs and further characterization of DEPs and genes will help in better understanding of the molecular events during sheath blight disease development in rice. (Copyright © 2018 Elsevier Masson SAS. All rights reserved.) |
Databáze: | MEDLINE |
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