Optimizing the Performance of Supported Lipid Bilayers as Cell Culture Platforms Based on Extracellular Matrix Functionalization.

Autor: Vafaei S; School of Materials Science and Engineering, Nanyang Technological University, 50 Nanyang Avenue, 639798 Singapore.; Centre for Biomimetic Sensor Science, Nanyang Technological University, 50 Nanyang Drive, 637553 Singapore., Tabaei SR; School of Materials Science and Engineering, Nanyang Technological University, 50 Nanyang Avenue, 639798 Singapore.; Centre for Biomimetic Sensor Science, Nanyang Technological University, 50 Nanyang Drive, 637553 Singapore., Cho NJ; School of Materials Science and Engineering, Nanyang Technological University, 50 Nanyang Avenue, 639798 Singapore.; Centre for Biomimetic Sensor Science, Nanyang Technological University, 50 Nanyang Drive, 637553 Singapore.; School of Chemical and Biomedical Engineering, Nanyang Technological University, 62 Nanyang Drive, 637459 Singapore.
Jazyk: angličtina
Zdroj: ACS omega [ACS Omega] 2017 Jun 30; Vol. 2 (6), pp. 2395-2404. Date of Electronic Publication: 2017 Jun 01.
DOI: 10.1021/acsomega.7b00158
Abstrakt: Strategies to fabricate biofunctionalized surfaces are essential for many biotechnological applications. Zwitterionic lipid bilayer coatings doped with lipids with chemically selective headgroups provide a robust platform for immobilization of biomolecules in an antifouling, protein resistant background. Herein, we assess the biological activity of two important components of the extracellular matrix (ECM), collagen type I (Col I) and fibronectin (FN), which are covalently attached to a supported lipid bilayer (SLB), and compare their activity with the same proteins, nonspecifically adsorbed onto a SiO 2 surface. The characterization of protein coatings by quartz crystal microbalance with dissipation revealed that Col I and FN attached to SLB are less dense and have higher structural flexibility than when adsorbed onto SiO 2 . Cell adhesion, proliferation, and function, as well as Col I-FN interactions, were more efficient on the ECM-functionalized SLB, making it a promising platform for cell-based diagnostics, tissue engineering, medical implants, and biosensor development.
Competing Interests: The authors declare no competing financial interest.
Databáze: MEDLINE