The effect of Sebastiania hispida gel on wound model infected by methicillin resistant Staphylococcus aureus.

Autor: Muller JAI; Postgraduate Program in Biochemistry and Molecular Biology, Federal University of Mato Grosso do Sul, Av. Costa and Silva, s/n - University City, 79070-900, Campo Grande, MS, Brazil. Electronic address: jessicaiamuller@gmail.com., Matias R; Postgraduate Program in Environment and Regional Development, University Anhanguera-Uniderp, Campo Grande, MS, Brazil., Guilhermino JF; Health and Environment: Biodiversity Program at Oswaldo Cruz Foundation MS, Campo Grande, MS, Brazil., Moreira DL; Health and Environment: Biodiversity Program at Oswaldo Cruz Foundation MS, Campo Grande, MS, Brazil., Dos Santos KS; Postgraduate Program in Environment and Regional Development, University Anhanguera-Uniderp, Campo Grande, MS, Brazil., Fermiano MH; Laboratory of Toxinology and Medicinal Plants, University Anhanguera-Uniderp, Campo Grande, MS, Brazil., Silva BAK; Federal University of Piauí, Parnaíba Campus, Parnaíba, PI, Brazil., Dourado DM; Laboratory of Toxinology and Medicinal Plants, University Anhanguera-Uniderp, Campo Grande, MS, Brazil; Postgraduate Program in Infectious and Parasitic Diseases, Federal University of Mato Grosso do Sul, Campo Grande, MS, Brazil.
Jazyk: angličtina
Zdroj: Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie [Biomed Pharmacother] 2018 Sep; Vol. 105, pp. 1311-1317. Date of Electronic Publication: 2018 Jun 23.
DOI: 10.1016/j.biopha.2018.06.100
Abstrakt: Medicinal plants are an alternative for the treatment of infected wounds. The objective of this study was to evaluate the efficacy of Sebastiania hispida in an animal model with a wound infected by Staphylococcus aureus. The crude ethanol extract (Ext EtOH ) of S. hispida underwent phytochemical analysis, quantification of metabolites and antibacterial activity analysis performed using S. aureus. Wistar rats were used to test healing activity, and the groups evaluated comprised gels of Ext EtOH at the concentrations 0.2 and 2% compared with control groups. Animals were inoculated with the bacteria S. aureus resistant to methicillin. The treatment periods were of 3 and 21 days. Macroscopic and microscopic analysis were conducted and data were submitted to analysis of variance (p < 0.05). Phytochemical and quantification analysis indicated that phenolic compounds and flavonoids are the major constituents, followed by tyterpenes. Ext EtOH 0.2% was the most effective gel against the growth of strains of S. aureus. Histological wound and regression analysis showed that Ext EtOH gels (0.2% and 2%) were similar and effective in promoting wound healing. In the quantification of collagen fibers, the animals from all groups showed a high amount of thick collagen fibers. Thus, Ext EtOH gels based on the shoots of S. hispida can be used for the treatment of infected wounds as a complementary therapy for infected wound closure and further assays are required with other means. The healing effectiveness may be due to the high content of phenolics, flavonoids and triterpenes.
(Copyright © 2018 Elsevier Masson SAS. All rights reserved.)
Databáze: MEDLINE
Externí odkaz: