In vitro e in silico evaluation of the inhibition of Staphylococcus aureus efflux pumps by caffeic and gallic acid.

Autor: Dos Santos JFS; Laboratory of Semi-Arid Bioprospecting (Lab-Bioprospec), Department of Biological Chemistry/CCBS/URCA, Ceará, Brazil. Electronic address: joycy.sampaio22@gmail.com., Tintino SR; Laboratory of Microbiology and Molecular Biology (LMBM), Department of BiologicalChemistry/CCBS/URCA, Ceará, Brazil. Electronic address: saulorelison@gmail.com., de Freitas TS; Laboratory of Microbiology and Molecular Biology (LMBM), Department of BiologicalChemistry/CCBS/URCA, Ceará, Brazil. Electronic address: thiagocrato@hotmail.com., Campina FF; Laboratory of Microbiology and Molecular Biology (LMBM), Department of BiologicalChemistry/CCBS/URCA, Ceará, Brazil. Electronic address: fabiacampina@gmail.com., de A Menezes IR; Laboratory of Pharmacology and Molecular Chemistry (LFQM), Department of Biological Chemistry/CCBS/URCA, Ceará, Brazil. Electronic address: irwinalencar@yahoo.com.br., Siqueira-Júnior JP; Laboratory of Microrganisms Genetics (LGM), Department of Molecular Biology/CCEN/UFPB, Paraíba, Brazil. Electronic address: jpsiq@uol.com.br., Coutinho HDM; Laboratory of Microbiology and Molecular Biology (LMBM), Department of BiologicalChemistry/CCBS/URCA, Ceará, Brazil. Electronic address: hdmcoutinho@urca.br., Cunha FAB; Laboratory of Semi-Arid Bioprospecting (Lab-Bioprospec), Department of Biological Chemistry/CCBS/URCA, Ceará, Brazil. Electronic address: cunha.urca@gmail.com.
Jazyk: angličtina
Zdroj: Comparative immunology, microbiology and infectious diseases [Comp Immunol Microbiol Infect Dis] 2018 Apr; Vol. 57, pp. 22-28. Date of Electronic Publication: 2018 Mar 12.
DOI: 10.1016/j.cimid.2018.03.001
Abstrakt: Staphylococcus aureus has been reported as one of the most difficult to treat. In the search for new treatment alternatives, isolated plant substances such as phenolic compounds, have demonstrated the ability to reverse bacterial resistance. The present study aims to evaluate the inhibitory action of caffeic acid and gallic acid on efflux pumps from S. aureus resistant strains. The broth microdilution assay was carried out to obtain the MICs of caffeic acid and gallic acid while the efflux pump inhibition test was assessed through the reduction of the minimum inhibitory concentration of the antibiotic and ethidium bromide. In addition, in silico theoretical parameters were analyzed to determine the theoretical efficacy of the compound and its free energy of interaction. In the results, the inhibition concentration of the two compounds did not certify clinical relevance with 1024 μg/mL for all strains. In the efflux pump inhibition effect, caffeic acid inhibited the MrsA pumps of the strain RN-4220 and NorA of the strain 1199B. Caffeic acid showed greater efficacy in the docking model, in agreement with the demonstrated experimental efficacy. Isolated compounds can be indicated as efficient options in the inhibition of resistance mechanisms.
(Copyright © 2018 Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
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