Volcanic ash from Puyehue-Cordón Caulle Volcanic Complex and Calbuco promote a differential response of pro-inflammatory and oxidative stress mediators on human conjunctival epithelial cells.

Autor: Tesone AI; University of Buenos Aires, School of Medicine, Pathology Department, Ocular Investigation Laboratory, Buenos Aires, Argentina. Electronic address: atesone10@gmail.com., Lasagni Vitar RM; University of Buenos Aires, Faculty of Pharmacy and Biochemistry, General and Inorganic Chemistry Division, Buenos Aires, Argentina; CONICET - University of Buenos Aires, Instituto de Bioquímica y Medicina Molecular (IBIMOL), Buenos Aires, Argentina; University of Buenos Aires, School of Pharmacy and Biochemistry, Analytical Chemistry and Phisicochemistry Department, General and Inorganic Chemistry Division, Buenos Aires, Argentina., Tau J; University of Buenos Aires, School of Medicine, Pathology Department, Ocular Investigation Laboratory, Buenos Aires, Argentina., Maglione GA; National University of San Martín, School of Science and Technology, Buenos Aires, Argentina; University of Buenos Aires, School of Dentistry, Department of Histology and Embryology, Buenos Aires, Argentina., Llesuy S; University of Buenos Aires, Faculty of Pharmacy and Biochemistry, General and Inorganic Chemistry Division, Buenos Aires, Argentina; CONICET - University of Buenos Aires, Instituto de Bioquímica y Medicina Molecular (IBIMOL), Buenos Aires, Argentina; University of Buenos Aires, School of Pharmacy and Biochemistry, Analytical Chemistry and Phisicochemistry Department, General and Inorganic Chemistry Division, Buenos Aires, Argentina., Tasat DR; National University of San Martín, School of Science and Technology, Buenos Aires, Argentina; University of Buenos Aires, School of Dentistry, Department of Histology and Embryology, Buenos Aires, Argentina., Berra A; University of Buenos Aires, School of Medicine, Pathology Department, Ocular Investigation Laboratory, Buenos Aires, Argentina.
Jazyk: angličtina
Zdroj: Environmental research [Environ Res] 2018 Nov; Vol. 167, pp. 87-97. Date of Electronic Publication: 2018 Jul 07.
DOI: 10.1016/j.envres.2018.07.013
Abstrakt: Volcanic ash could pose a hazard to the ocular surface as it is constantly exposed to environmental particles. We exposed conjunctival cells to Puyehue-Cordón Caulle volcanic complex (PCCVC) or Calbuco ash particles and evaluated proliferation, viability, apoptosis, MUC1 expression, pro-inflammatory cytokines, and oxidative stress markers. Ash particles from these volcanoes vary in size, composition, and morphology. Our results demonstrate that PCCVC but not Calbuco ash particles induce cytotoxicity on human conjunctival epithelial cells viewed as a decrease in cell proliferation and the transmembrane mucin MUC1 expression; a pro-inflammatory response mediated by IL-6 and IL-8; and an imbalance of the redox environment leading to protein oxidative damage. This is the first in vitro study that assesses the biological effect of volcanic ash particles on human conjunctival epithelial cells and the involvement of inflammatory mediators and oxidative stress as the mechanisms of damage. Our results could provide a better understanding of the ocular symptoms manifested by people living near volcanic areas.
(Copyright © 2018 Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
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