[Gender-dependent Expression of ERβ in AEC Ⅱ of Fetal Mice Exposed to Arsenic and Estrogen Receptor Antagonist].

Autor: Yang MP; Department of Environmental Health and Occupational Medicine,West China School of Public Health,Sichuan University,Chengdu 610041,China., Che WJ; Center for Disease Control and Prevention of Kunming,Kunming 650022,China., Cheng YL; Department of Environmental Health and Occupational Medicine,West China School of Public Health,Sichuan University,Chengdu 610041,China., Xiong LS; Department of Environmental Health and Occupational Medicine,West China School of Public Health,Sichuan University,Chengdu 610041,China., Zhang H; Department of Environmental Health and Occupational Medicine,West China School of Public Health,Sichuan University,Chengdu 610041,China.
Jazyk: čínština
Zdroj: Sichuan da xue xue bao. Yi xue ban = Journal of Sichuan University. Medical science edition [Sichuan Da Xue Xue Bao Yi Xue Ban] 2018 May; Vol. 49 (3), pp. 364-368.
Abstrakt: Objective: To determine the effects of arsenic and estrogen receptor antagonist (ICI182, 780) on the expression of estrogen receptor beta (ERβ) in alveolar Ⅱ epithelial cells (AECⅡ) of female and male mice.
Methods: Nineteen or twenty day fetus mice were obtained through caesarean section of ICR mice. Purified AECⅡ cells were separated from the female and male fetus,respectively,and confirmed using immunofluorescence staining. The cells were exposed to sodium arsenite (NaAsO 2 ) at a low,medium,or high dosage determined by MTT and cultured for 24 h. The NaAsO 2 (5 μmol/L) exposed cells were compared with those treated (for 24 h) with dimethyl sulfoxide (DMSO) or ICI182, 780 (1×10 -4 mol/L). Apoptosis rates of the cells were measured by flow cytometry. Real-time fluorescence quantitative PCR method and Western blot technique were used to detect the expression of ERβ mRNA and protein in AECⅡ.
Results: Purity of AECⅡ cells reached (87.0±2.5)%. NaAsO 2 exposure was set at a concentration of 0.5 (low),1.25 (medium),and 5 (high) μmol/L. The cells exposed to medium and high dosage of NaAsO 2 had higher apoptosis rates than the blank controls ( P <0.05),without sex differences. Female cells exposed to medium and high dosage of NaAsO 2 had higher levels of expressions of ERβ mRNA and protein than the blank controls ( P <0.05) and male cells exposed to the same dosage of NaAsO 2 ( P <0.05). No significant differences were found in the expressions of ERβ mRNA and protein between the exposed male cells and the blank controls. ICI182, 780 lowered the expression levels of ERβ mRNA and protein in the female exposed cells ( P <0.01).
Conclusion: Arsenic exposure increases expressions of AECⅡ's ERβ,more so in female cells than in male cells. This can be blocked by estrogen receptor antagonists.
(Copyright© by Editorial Board of Journal of Sichuan University (Medical Science Edition).)
Databáze: MEDLINE
Externí odkaz: