In Vitro Studies of the Antimicrobial and Free-Radical Scavenging Potentials of Silver Nanoparticles Biosynthesized From the Extract of Desmostachya bipinnata .

Autor: Guntur SR; Department of Biomedical Engineering, Vignan's Foundation for Science, Technology & Research (Deemed to be University), Guntur, India., Kumar NS; Department of Biotechnology, Vignan's Foundation for Science, Technology & Research (Deemed to be University), Guntur, India., Hegde MM; Department of Nanotechnology, Rajiv Gandhi Institute of Technology, Bangalore, India., Dirisala VR; Department of Biotechnology, Vignan's Foundation for Science, Technology & Research (Deemed to be University), Guntur, India.
Jazyk: angličtina
Zdroj: Analytical chemistry insights [Anal Chem Insights] 2018 Jul 02; Vol. 13, pp. 1177390118782877. Date of Electronic Publication: 2018 Jul 02 (Print Publication: 2018).
DOI: 10.1177/1177390118782877
Abstrakt: The aim of this study was to perform green synthesis of silver nanoparticles (AgNPs) from the leaf extract of Desmostachya bipinnata (Dharba), a medicinally important herb which is widely used across India. Synthesized AgNPs were analyzed by UV-Visible spectroscopy, X-ray diffraction (XRD), Fourier transform infrared (FTIR) spectroscopy, scanning electron microscopy (SEM), and energy dispersive X-ray spectroscopy (EDAX). The results have confirmed that green synthesis of AgNPs leads to the fabrication of sphere-shaped particles with a diameter of 53 nm. Furthermore, these AgNPs were subjected to antioxidant and antimicrobial studies against gram-negative and gram-positive bacteria, where AgNPs at a concentration of 20 mg/mL showed highest zone of inhibition. Synthesized AgNPs were evaluated for their antioxidant activity by 1, 1-diphenyl-2-picryl hydrazyl radical (DPPH), H 2 O 2 , and superoxide inhibiting assays; increasing concentration has showed increase in scavenging ability. Cell toxicity was assessed on HepG2 cell lines, and synthesized nanoparticles at a concentration of 128 μg/mL produced significant reduction in viability of Hep cells ( P  < .05). The availability of Dharba throughout the year and the eco-friendly approach in the synthesis of AgNPs coupled with bioactivity has demonstrated its potential as a novel biomaterial which can be used for various biomedical applications.
Competing Interests: Declaration of conflicting interests:The author(s) declared no potential conflicts of interest with respect to the research, authorship, and/or publication of this article.
Databáze: MEDLINE
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