MicroRNA-23a/27a/24-2 cluster promotes gastric cancer cell proliferation synergistically.

Autor: Hua K; Department and Institute of Pharmacology, School of Medicine, National Yang-Ming University, Taipei 11221, Taiwan, R.O.C.; VYM Genome Research Center, School of Medicine, National Yang-Ming University, Taipei 11221, Taiwan, R.O.C., Chen YT; Department and Institute of Pharmacology, School of Medicine, National Yang-Ming University, Taipei 11221, Taiwan, R.O.C., Chen CF; VYM Genome Research Center, School of Medicine, National Yang-Ming University, Taipei 11221, Taiwan, R.O.C., Tang YS; Department and Institute of Pharmacology, School of Medicine, National Yang-Ming University, Taipei 11221, Taiwan, R.O.C., Huang TT; Department and Institute of Pharmacology, School of Medicine, National Yang-Ming University, Taipei 11221, Taiwan, R.O.C.; Department of Anatomy and Cell Biology, School of Medicine, National Yang-Ming University, Taipei 11221, Taiwan, R.O.C., Lin YC; Department and Institute of Pharmacology, School of Medicine, National Yang-Ming University, Taipei 11221, Taiwan, R.O.C., Yeh TS; Department and Institute of Pharmacology, School of Medicine, National Yang-Ming University, Taipei 11221, Taiwan, R.O.C.; Department of Anatomy and Cell Biology, School of Medicine, National Yang-Ming University, Taipei 11221, Taiwan, R.O.C.; Institute of Biochemistry and Molecular Biology, National Yang-Ming University, Taipei 11221, Taiwan, R.O.C.; Graduate Institute of Medical Sciences, College of Medicine, Taipei Medical University, Taipei 11031, Taiwan, R.O.C., Huang KH; Department of Surgery, Taipei Veterans General Hospital, Taipei 11221, Taiwan, R.O.C.; Institute of Clinical Medicine, School of Medicine, National Yang-Ming University, Taipei 11221, Taiwan, R.O.C., Lee HC; Department and Institute of Pharmacology, School of Medicine, National Yang-Ming University, Taipei 11221, Taiwan, R.O.C., Hsu MT; VYM Genome Research Center, School of Medicine, National Yang-Ming University, Taipei 11221, Taiwan, R.O.C.; Institute of Biochemistry and Molecular Biology, National Yang-Ming University, Taipei 11221, Taiwan, R.O.C., Chi CW; Department and Institute of Pharmacology, School of Medicine, National Yang-Ming University, Taipei 11221, Taiwan, R.O.C.; Department of Medical Research, Taipei Veterans General Hospital, Taipei 11221, Taiwan, R.O.C., Wu CW; Department of Surgery, Taipei Veterans General Hospital, Taipei 11221, Taiwan, R.O.C., Lin CH; VYM Genome Research Center, School of Medicine, National Yang-Ming University, Taipei 11221, Taiwan, R.O.C.; Institute of Microbiology and Immunology, National Yang-Ming University, Taipei 11221, Taiwan, R.O.C.; Institute of Biophotonics, National Yang-Ming University, Taipei 11221, Taiwan, R.O.C., Ping YH; Department and Institute of Pharmacology, School of Medicine, National Yang-Ming University, Taipei 11221, Taiwan, R.O.C.; VYM Genome Research Center, School of Medicine, National Yang-Ming University, Taipei 11221, Taiwan, R.O.C.; Institute of Biophotonics, National Yang-Ming University, Taipei 11221, Taiwan, R.O.C.
Jazyk: angličtina
Zdroj: Oncology letters [Oncol Lett] 2018 Aug; Vol. 16 (2), pp. 2319-2325. Date of Electronic Publication: 2018 Jun 07.
DOI: 10.3892/ol.2018.8924
Abstrakt: Previous studies have indicated that certain microRNAs (miRNAs/miRs) function as either tumor suppressors or oncogenes in human cancer. The present study identified the miR-23a/27a/24-2 cluster, containing miR-23, miR-27a and miR-24, as an oncogene in gastric cancer. The expression of the miR-23a/27a/24-2 cluster was upregulated in clinical gastric cancer tissues. Transfection with inhibitors of miR-23a, miR-27a, or miR-24, either independently or together, repressed in vitro colony formation and in vivo tumor formation. The miR23a/27a/24-2 cluster inhibitors repressed the growth of gastric cancer cells in a synergistic manner. In addition, treatment with lower doses of the miRNA inhibitor mixture induced the formation of apoptotic bodies. According to computational predictions using TargetScan, suppressor of cytokine-induced signaling 6 (SOCS6) was identified as one of the downstream target genes of the miR-23a/27a/24-2 cluster. The expression of SOCS6 was significantly lower in tumor tissues than in matched normal tissues (P<0.01) and was associated with poor survival (P<0.00001). Taken together, these results strongly suggested that the miR-23a/27a/24-2 cluster may mediate the progression of gastric cancer through the suppression of SOCS6 expression. The present study also provides a novel molecular target for the development of an anti-gastric cancer agent.
Databáze: MEDLINE
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