Transcriptomics investigation of thyroid hormone disruption in the olfactory system of the Rana [Lithobates] catesbeiana tadpole.

Autor: Jackman KW; Department of Biochemistry and Microbiology, University of Victoria, Victoria, British Columbia, V8P 5C2, Canada., Veldhoen N; Department of Biochemistry and Microbiology, University of Victoria, Victoria, British Columbia, V8P 5C2, Canada., Miliano RC; Environment and Climate Change Canada, Pacific Environmental Science Centre, 2645 Dollarton Highway, North Vancouver, British Columbia, V7H 1V2, Canada., Robert BJ; Department of Mathematics and Statistics, University of Victoria, Victoria, British Columbia, V8P 5C2, Canada., Li L; Department of Civil and Environmental Engineering, University of Waterloo, Waterloo, Ontario, N2L 3G1, Canada., Khojasteh A; Department of Civil Engineering, University of British Columbia, Vancouver, British Columbia, V6T 1Z4, Canada., Zheng X; Department of Civil Engineering, University of British Columbia, Vancouver, British Columbia, V6T 1Z4, Canada., Zaborniak TSM; Department of Biochemistry and Microbiology, University of Victoria, Victoria, British Columbia, V8P 5C2, Canada., van Aggelen G; Department of Mathematics and Statistics, University of Victoria, Victoria, British Columbia, V8P 5C2, Canada., Lesperance M; Department of Mathematics and Statistics, University of Victoria, Victoria, British Columbia, V8P 5C2, Canada., Parker WJ; Department of Civil and Environmental Engineering, University of Waterloo, Waterloo, Ontario, N2L 3G1, Canada., Hall ER; Department of Civil Engineering, University of British Columbia, Vancouver, British Columbia, V6T 1Z4, Canada., Pyle GG; Department of Biological Sciences, University of Lethbridge, Lethbridge, Alberta, T1K 3M4, Canada., Helbing CC; Department of Biochemistry and Microbiology, University of Victoria, Victoria, British Columbia, V8P 5C2, Canada. Electronic address: chelbing@uvic.ca.
Jazyk: angličtina
Zdroj: Aquatic toxicology (Amsterdam, Netherlands) [Aquat Toxicol] 2018 Sep; Vol. 202, pp. 46-56. Date of Electronic Publication: 2018 Jun 30.
DOI: 10.1016/j.aquatox.2018.06.015
Abstrakt: Thyroid hormones (THs) regulate vertebrate growth, development, and metabolism. Despite their importance, there is a need for effective detection of TH-disruption by endocrine disrupting chemicals (EDCs). The frog olfactory system substantially remodels during TH-dependent metamorphosis and the objective of the present study is to examine olfactory system gene expression for TH biomarkers that can evaluate the biological effects of complex mixtures such as municipal wastewater. We first examine classic TH-response gene transcripts using reverse transcription-quantitative real-time polymerase chain reaction (RT-qPCR) in the olfactory epithelium (OE) and olfactory bulb (OB) of premetamorphic Rana (Lithobates) catesbeiana tadpoles after 48 h exposure to biologically-relevant concentrations of the THs, 3,5,3'-triiodothyronine (T 3 ) and L-thyroxine (T 4 ), or 17-beta estradiol (E 2 ); a hormone that can crosstalk with THs. As the OE was particularly sensitive to THs, further RNA-seq analysis found >30,000 TH-responsive contigs. In contrast, E 2 affected 267 contigs of which only 57 overlapped with THs suggesting that E 2 has limited effect on the OE at this developmental phase. Gene ontology enrichment analyses identified sensory perception and nucleoside diphosphate phosphorylation as the top affected terms for THs and E 2 , respectively. Using classic and additional RNA-seq-derived TH-response gene transcripts, we queried TH-disrupting activity in municipal wastewater effluent from two different treatment systems: anaerobic membrane bioreactor (AnMBR) and membrane enhanced biological phosphorous removal (MEBPR). While we observed physical EDC removal in both systems, some TH disruption activity was retained in the effluents. This work lays an important foundation for linking TH-dependent gene expression with olfactory system function in amphibians.
(Copyright © 2018 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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