Influences of maternal nutrient restriction and arginine supplementation on visceral metabolism and hypothalamic circuitry of offspring.

Autor: Prezotto LD; Department of Animal Sciences, North Dakota State University, Hultz Hall 166, Dept. 7630, PO Box 6050, Fargo 58108, USA; Northern Agricultural Research Center, Montana State University, 3710 Assinniboine Road, Havre 59501, USA., Thorson JF; Northern Agricultural Research Center, Montana State University, 3710 Assinniboine Road, Havre 59501, USA; USDA, ARS, U.S. Meat Animal Research Center, P.O. Box 166, Clay Center 68933, USA., Borowicz PP; Department of Animal Sciences, North Dakota State University, Hultz Hall 166, Dept. 7630, PO Box 6050, Fargo 58108, USA., Peine JL; Department of Animal Sciences, North Dakota State University, Hultz Hall 166, Dept. 7630, PO Box 6050, Fargo 58108, USA., Bedenbaugh M; Department of Physiology & Pharmacology, West Virginia University, Morgantown, WV 26506, USA., Hileman SM; Department of Physiology & Pharmacology, West Virginia University, Morgantown, WV 26506, USA., Lents CA; USDA, ARS, U.S. Meat Animal Research Center, P.O. Box 166, Clay Center 68933, USA., Caton JS; Department of Animal Sciences, North Dakota State University, Hultz Hall 166, Dept. 7630, PO Box 6050, Fargo 58108, USA., Swanson KC; Department of Animal Sciences, North Dakota State University, Hultz Hall 166, Dept. 7630, PO Box 6050, Fargo 58108, USA. Electronic address: kendall.swanson@ndsu.edu.
Jazyk: angličtina
Zdroj: Domestic animal endocrinology [Domest Anim Endocrinol] 2018 Oct; Vol. 65, pp. 71-79. Date of Electronic Publication: 2018 Jun 20.
DOI: 10.1016/j.domaniend.2018.06.001
Abstrakt: Maternal nutrient restriction during gestation can exert long-term negative effects on offspring health and performance. Arginine supplementation may rescue some of the negative effects elicited by maternal nutrient restriction. We tested the hypothesis that maternal arginine supplementation during gestation would rescue deleterious effects of nutrient restriction on in vitro O 2 consumption in the liver and jejunum and hypothalamic protein expression of proopiomelanocortin (POMC), neuropeptide Y (NPY), agouti-related peptide (AgRP), and neuronal nitric oxide synthase (nNOS), and the colocalization of nNOS and active phosphor-signal transducer and activator of transcription 3 (pSTAT3) in female offspring. Multiparous ewes were assigned to dietary treatment at 54 d of gestation: 100% of requirements (Con), 60% of control (Res), or Res plus rumen-protected arginine (Res-Arg; 180 mg/kg). At parturition, offspring were immediately removed from their dam and placed on a common diet. At 54 ± 4 d of age, female lambs (n = 6 per treatment) were weighed, the liver and jejunum were weighed, and samples were collected for in vitro measurement of O 2 consumption. The hypothalamus was collected to determine protein expression of POMC, NPY, AgRP, and nNOS, and the colocalization of nNOS and pSTAT3 (n = 3, 4, and 4 for Con, Res, and Res-Arg, respectively). Hepatic consumption of O 2 in vitro (mol/min/liver) was decreased (P = 0.04) in the Res and Res-Arg group compared with Con. Intensity of staining for NPY-containing fibers tended to decrease (P = 0.10) in Res and Res-Arg compared with Con. Number of POMC neuronal cells in the arcuate nucleus (ARC) of the hypothalamus decreased (P ≤ 0.03) in the Res group compared with Res-Arg. These observations demonstrate that maternal nutrient restriction decreases energy utilization in the liver and number of POMC cells in the ARC of offspring. Supplementation of arginine to the gestating ewe failed to influence hepatic use of energy in lambs from Res ewes. Numbers of POMC-containing cells were increased in the ARC in lambs from ewes restricted to 60% of nutritional requirements and supplemented with rumen-protected arginine, potentially influencing feeding behavior and hepatic energy metabolism.
(Copyright © 2018 Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
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