| Autor: |
Olajuyin AM; State Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Beijing, People's Republic of China.; University of Chinese Academy of Sciences, Beijing, 100049, People's Republic of China., Yang M; State Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Beijing, People's Republic of China., Mu T; State Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Beijing, People's Republic of China.; University of Chinese Academy of Sciences, Beijing, 100049, People's Republic of China., Tian J; State Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Beijing, People's Republic of China.; University of Chinese Academy of Sciences, Beijing, 100049, People's Republic of China., Thygesen A; Department of Chemical and Biochemical Engineering, Technical University of Denmark, 2800, Lyngby, Denmark.; Sino-Danish Center for Education and Research, Niels Jensensvej 2, 8000, Aarhus C, Denmark., Adesanoye OA; Department of Biochemistry, Faculty of Basic Medical Sciences, University of Ibadan, Ibadan, Nigeria., Adaramoye OA; Department of Biochemistry, Faculty of Basic Medical Sciences, University of Ibadan, Ibadan, Nigeria., Song A; Key Laboratory of Enzyme Engineering of Agricultural Microbiology, Ministry of Agriculture, College of Life Sciences, Henan Agricultural University, Zhengzhou, 450002, People's Republic of China. Song1666@126.com., Xing J; State Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Beijing, People's Republic of China. jmxing@ipe.ac.cn.; University of Chinese Academy of Sciences, Beijing, 100049, People's Republic of China. jmxing@ipe.ac.cn. |
| Abstrakt: |
A biorefinery process for high yield production of succinic acid from biomass sugars was investigated using recombinant Escherichia coli. The major problem been addressed is utilization of waste biomass for the production of succinic acid using metabolic engineering strategy. Here, methanol extract of Strophanthus preussii was used for fermentation. The process parameters were optimized. Glucose (9 g/L), galactose (4 g/L), xylose (6 g/L) and arabinose (0.5 g/L) were the major sugars present in the methanol extract of S. preussii. E. coli K3OS with overexpression of soluble nucleotide pyridine transhydrogenase sthA and mutation of lactate dehydrogenase A (ldhA), phosphotransacetylase acetate kinase A (pta-ackA), pyruvate formate lyase B (pflB), pyruvate oxidase B (poxB), produced a final succinic acid concentration of 14.40 g/L and yield of 1.10 mol/mol total sugars after 72 h dual-phase fermentation in M9 medium. Here, we show that the maximum theoretical yield using methanol extracts of S. preussii was 64%. Hence, methanol extract of S. preussii could be used for the production of biochemicals such as succinate, malate and pyruvate. |
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