The protective effect of berberine against lipopolysaccharide-induced abortion by modulation of inflammatory/immune responses.

Autor: Mahdavi Siuki M; a Department of Animal Biotechnology, National Institute of Genetic Engineering and Biotechnology , Tehran , Iran., Fazel Nasab N; b Department of English Language , Eghbal Lahoori Institute of Higher Education , Mashhad , Iran., Barati E; c Department of Biology , Payame Noor University of Mashhad , Mashhad , Iran., Nezafat Firizi M; d Department of Pathobiology and Laboratory Sciences , North Khorasan University of Medical Sciences , Bojnurd , Iran., Jalilvand T; e Department of Molecular Science, School of Medicine , North Khorasan University of Medical Sciences , Bojnurd , Iran., Namdar Ahmadabad H; d Department of Pathobiology and Laboratory Sciences , North Khorasan University of Medical Sciences , Bojnurd , Iran.
Jazyk: angličtina
Zdroj: Immunopharmacology and immunotoxicology [Immunopharmacol Immunotoxicol] 2018 Aug; Vol. 40 (4), pp. 333-337. Date of Electronic Publication: 2018 Jul 13.
DOI: 10.1080/08923973.2018.1485155
Abstrakt: Objective: Berberine is an isoquinoline derivative alkaloid with anti-inflammatory activity. In this study, we investigated the protective effects of berberine in prevention of LPS-induced abortion.
Materials and Methods: On the gestation day (GD) 9.5, the pregnant mice were injected with low, medium, and high doses of berberine or with PBS. After 4 h, berberine or PBS-pretreated mice were injected with LPS. On GD 11.5, blood samples and uterine tissues were collected from treated mice and percentage of abortion and serum levels of NO, TNF-α, IL-10, and IL12p70 were measured by macroscopic examination and sandwich ELISA, respectively.
Results: Our findings show that mice injected with berberine were resistant to LPS-induced abortion. We also found that this treatment prevents the reduction of IL-10 and the enhancement of NO, TNF-α, and IL-12p70 in LPS-treated pregnant mice.
Conclusions: Taken together, our results suggest that berberine as an anti-inflammatory agent has protective effects on LPS-induced abortion by modulation of inflammatory/immune responses.
Databáze: MEDLINE
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