Characterized non-transient microbiota from stinkbug (Nezara viridula) midgut deactivates soybean chemical defenses.

Autor: Medina V; Universidad de Buenos Aires, Facultad de Agronomía, Cátedra de Bioquímica -Instituto de Investigaciones en Biociencias Agrícolas y Ambientales (INBA-CONICET), Buenos Aires, Argentina., Sardoy PM; Universidad de Buenos Aires, Facultad de Agronomía, Cátedra de Bioquímica -Instituto de Investigaciones en Biociencias Agrícolas y Ambientales (INBA-CONICET), Buenos Aires, Argentina., Soria M; Universidad de Buenos Aires, Facultad de Agronomía, Cátedra de Microbiología -Instituto de Investigaciones en Biociencias Agrícolas y Ambientales (INBA-CONICET), Buenos Aires, Argentina., Vay CA; Universidad de Buenos Aires, Facultad de Farmacia y Bioquímica, Buenos Aires, Argentina., Gutkind GO; Universidad de Buenos Aires, Facultad de Farmacia y Bioquímica, Buenos Aires, Argentina.; Consejo Nacional de Investigaciones Científicas y Técnicas de Argentina, (CONICET), Buenos Aires, Argentina., Zavala JA; Universidad de Buenos Aires, Facultad de Agronomía, Cátedra de Bioquímica -Instituto de Investigaciones en Biociencias Agrícolas y Ambientales (INBA-CONICET), Buenos Aires, Argentina.; Consejo Nacional de Investigaciones Científicas y Técnicas de Argentina, (CONICET), Buenos Aires, Argentina.
Jazyk: angličtina
Zdroj: PloS one [PLoS One] 2018 Jul 12; Vol. 13 (7), pp. e0200161. Date of Electronic Publication: 2018 Jul 12 (Print Publication: 2018).
DOI: 10.1371/journal.pone.0200161
Abstrakt: The Southern green stinkbug (N. viridula) feeds on developing soybean seeds in spite of their strong defenses against herbivory, making this pest one of the most harmful to soybean crops. To test the hypothesis that midgut bacterial community allows stinkbugs to tolerate chemical defenses of soybean developing seeds, we identified and characterized midgut microbiota of stinkbugs collected from soybean crops, different secondary plant hosts or insects at diapause on Eucalyptus trees. Our study demonstrated that while more than 54% of N. viridula adults collected in the field had no detectable bacteria in the V1-V3 midgut ventricles, the guts of the rest of stinkbugs were colonized by non-transient microbiota (NTM) and transient microbiota not present in stinkbugs at diapause. While transient microbiota Bacillus sp., Micrococcus sp., Streptomyces sp., Staphylococcus sp. and others had low abundance, NTM microbiota was represented by Yokenella sp., Pantoea sp. and Enterococcus sp. isolates. We found some isolates that showed in vitro β-glucosidase and raffinase activities plus the ability to degrade isoflavonoids and deactivate soybean protease inhibitors. Our results suggest that the stinkbugs´ NTM microbiota may impact on nutrition, detoxification and deactivation of chemical defenses, and Enterococcus sp., Yokenella sp. and Pantoea sp. strains might help stinkbugs to feed on soybean developing seeds in spite of its chemical defenses.
Competing Interests: The authors have declared that no competing interests exist.
Databáze: MEDLINE
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