Effects of the prebiotic mannan oligosaccharide on the experimental periodontitis in rats.

Autor: Levi YLAS; Graduate Program in Dentistry (GPD-Master's Degree), University of Western Sao Paulo-UNOESTE, Presidente Prudente, Sao Paulo, Brazil., Novais GS; Graduate Program in Dentistry (GPD-Master's Degree), University of Western Sao Paulo-UNOESTE, Presidente Prudente, Sao Paulo, Brazil., Dias RB; Graduate Program in Dentistry (GPD-Master's Degree), University of Western Sao Paulo-UNOESTE, Presidente Prudente, Sao Paulo, Brazil., Andraus RAC; Graduate Program in Rehabilitation Sciences, Londrina, University Pitagoras UNOPAR Parana, Brazil., Messora MR; Departament of Oral and Maxillofacial Surgery and Periodontology, School of Dentistry of Ribeirão Preto, University of Sao Paulo-USP, Ribeirao Preto, Sao Paulo, Brazil., Neto HB; Department of Functional Sciences, University of Western Sao Paulo-UNOESTE, Presidente Prudente, Sao Paulo, Brazil., Ervolino E; Division of Histology, Department of Basic Sciences, Dental School of Aracatuba, University Estadual Paulista-UNESP, Aracatuba, Sao Paulo, Brazil., Santinoni CS; Graduate Program in Dentistry (GPD-Master's Degree), University of Western Sao Paulo-UNOESTE, Presidente Prudente, Sao Paulo, Brazil., Maia LP; Graduate Program in Dentistry (GPD-Master's Degree), University of Western Sao Paulo-UNOESTE, Presidente Prudente, Sao Paulo, Brazil.; Graduate Program in Dentistry, University Pitagoras UNOPAR, Londrina, Parana, Brazil.
Jazyk: angličtina
Zdroj: Journal of clinical periodontology [J Clin Periodontol] 2018 Sep; Vol. 45 (9), pp. 1078-1089. Date of Electronic Publication: 2018 Aug 20.
DOI: 10.1111/jcpe.12987
Abstrakt: Aim: To evaluate the effect of the prebiotic (PREB) mannan oligosaccharide (MOS) on the progression of the experimental periodontitis (EP) and intestinal morphology in rats.
Materials and Methods: Forty rats were randomly allocated into groups (n = 10): C (control), PREB, EP and EP-PREB. Groups PREB and EP-PREB received MOS incorporated into the feed daily. After 30 days, groups EP and EP-PREB received a cotton ligature around their mandibular first molars, kept for 14 days. Morphometrical, histomorphometrical, microcomputed tomography, gene expression analyses and immunohistochemistry were performed. Data were statistically analysed (p < 0.05).
Results: Group EP-PREB showed less interproximal bone loss, area without bone in the furcation and bone porosity, and greater bone mineral density than group EP (p < 0.05). It was also observed a significant decrease in IL-10 and IFN-γ gene expression, besides a decrease in TNF-α and IL-1β and an increase in TGF-β immunolabeling score for group EP-PREB. Group EP-PREB also presented villous height and crept depth values similar to group C, while group EP presented reduced values (p < 0.05).
Conclusion: It can be concluded that the oral administration of MOS promotes a protective effect against alveolar bone loss caused by EP in rats, modifying histologic and immune-inflammatory parameters, in addition to protecting the intestine.
(© 2018 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.)
Databáze: MEDLINE
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