[Differences in the cytocompatibility of bone-plastic materials from xenogeneic hydroxyapatite with stem cells from human exfoliated deciduous teeth and adipose tissue-derived mesenchymal stem cells].

Autor:	Vasilyev AV; Central Research Institute of Dentistry and Maxillofacial Surgery, Moscow, Russia; Research Centre for Medical Genetics, Moscow, Russia., Zorina OA; Central Research Institute of Dentistry and Maxillofacial Surgery, Moscow, Russia., Magomedov RN; Central Research Institute of Dentistry and Maxillofacial Surgery, Moscow, Russia., Bukharova TB; Research Centre for Medical Genetics, Moscow, Russia., Fatkhudinova NL; Central Research Institute of Dentistry and Maxillofacial Surgery, Moscow, Russia., Osidak EO; Imtek Ltd., Moscow, Russia., Domogatsky SP; Cardiology Research and Production Center Federal State Budgetary Institution, Ministry of Health of the Russian Federation, Moscow, Russia., Goldstein DV; Research Centre for Medical Genetics, Moscow, Russia.
Jazyk:	ruština
Zdroj:	Stomatologiia [Stomatologiia (Mosk)] 2018; Vol. 97 (3), pp. 7-13.
DOI:	10.17116/stomat20189737
Abstrakt:	Aim: To compare the cytocompatibility of osteoplastic materials used in dentistry with stem cells from human exfoliated deciduous teeth (SHED) and adipose tissue-derived mesenchymal stem cells (AD-MSC). Material and Methods: Materials of the brands 'Bio-Oss', 'Indost', 'Bioplast', 'Viscoll' and 'Trikafor' were selected for study purposes. Cultures of SHED and AD-MSC were used for testing. The cytotoxic effect of the materials was determined using MTT test and vital staining with trypan blue. Cell adhesion was assessed by the vital staining of PKH-26. Results: Water extracts of bone-plastic materials from xenogeneic hydroxyapatite of the brands 'Bio-Oss', 'Indost' and 'Bioplast' exert a cytotoxic effect on SHED and do not cause the death of AD-MSC. Materials based on collagen and β-tricalcium phosphate possess high cytocompatibility with all cell cultures under study. Conclusion: From the point of cytocompatibility all the examined bone-plastic materials may be considered safe for the restoration of bone defects. It should be noted that SHED transplantation on the surface of materials containing xenogeneic hydroxypatite is unacceptable.
Databáze:	MEDLINE
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