Metagenomic evaluation of the effects of storage conditions on the bacterial microbiota of oysters Crassostrea gasar (Adanson, 1757).

Autor: Ostrensky A; Integrated Group for Aquaculture and Environmental Studies, Federal University of Paraná, Curitiba, Paraná, Brazil.; Department of Zoology, Federal University of Paraná, Curitiba, Paraná, Brazil.; Department of Animal Science, Federal University of Paraná, Curitiba, Paraná, Brazil., Horodesky A; Integrated Group for Aquaculture and Environmental Studies, Federal University of Paraná, Curitiba, Paraná, Brazil.; Department of Zoology, Federal University of Paraná, Curitiba, Paraná, Brazil., Faoro H; Department of Biochemistry, Federal University of Paraná, Curitiba, Paraná, Brazil., Balsanelli E; Carlos Chagas Institut, Oswaldo Cruz Foundation, Curitiba, Paraná, Brazil., Sfeir MZT; Carlos Chagas Institut, Oswaldo Cruz Foundation, Curitiba, Paraná, Brazil., Cozer N; Integrated Group for Aquaculture and Environmental Studies, Federal University of Paraná, Curitiba, Paraná, Brazil.; Department of Animal Science, Federal University of Paraná, Curitiba, Paraná, Brazil., Pie MR; Department of Zoology, Federal University of Paraná, Curitiba, Paraná, Brazil., Dal Pont G; Integrated Group for Aquaculture and Environmental Studies, Federal University of Paraná, Curitiba, Paraná, Brazil.; Department of Animal Science, Federal University of Paraná, Curitiba, Paraná, Brazil., Castilho-Westphal GG; Integrated Group for Aquaculture and Environmental Studies, Federal University of Paraná, Curitiba, Paraná, Brazil.; Department of Zoology, Federal University of Paraná, Curitiba, Paraná, Brazil.
Jazyk: angličtina
Zdroj: Journal of applied microbiology [J Appl Microbiol] 2018 Nov; Vol. 125 (5), pp. 1435-1443. Date of Electronic Publication: 2018 Aug 30.
DOI: 10.1111/jam.14045
Abstrakt: Aims: To evaluate the influence of storage conditions on the composition of the bacterial microbiota of living oysters Crassostrea gasar.
Methods and Results: The oysters used in this study came from marine farms (Guaratuba Bay, Brazil) and were exposed to two conditions that simulated different storage situations: immersion in water (group I) and exposure to air (group II). The animals were subjected to five different temperatures (5-25°C), for 10 days. The 16S rRNA gene from oysters was amplified and sequenced to determine the taxonomic units and bacterial strains present in the samples. Group I showed higher diversity of bacteria (163 genera) rather than group II (104 genera). In all, 59 bacterial genera potentially pathogenic to humans were identified (n = 56 in group I and n = 45 in group II).
Conclusions: The storage conditions having a direct influence on the oyster microbiota. Live C. gasar should be stored exposed to air at 5-25°C, because it favours a lower prevalence of bacteria potentially pathogenic to humans.
Significance and Impact of the Study: During the oyster commercialization process, some conditions of storage, time and temperature must be followed in order to reduce the prevalence of bacteria potentially pathogenic to humans.
(© 2018 The Society for Applied Microbiology.)
Databáze: MEDLINE
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