Mass spectrometry-assisted venom profiling of Hypnale hypnale found in the Western Ghats of India incorporating de novo sequencing approaches.

Autor: Vanuopadath M; School of Biotechnology, Amrita Vishwa Vidyapeetham, Kollam 690 525, Kerala, India., Sajeev N; School of Biotechnology, Amrita Vishwa Vidyapeetham, Kollam 690 525, Kerala, India., Murali AR; School of Biotechnology, Amrita Vishwa Vidyapeetham, Kollam 690 525, Kerala, India., Sudish N; School of Biotechnology, Amrita Vishwa Vidyapeetham, Kollam 690 525, Kerala, India., Kangosseri N; School of Biotechnology, Amrita Vishwa Vidyapeetham, Kollam 690 525, Kerala, India., Sebastian IR; School of Biotechnology, Amrita Vishwa Vidyapeetham, Kollam 690 525, Kerala, India., Jain ND; School of Biotechnology, Amrita Vishwa Vidyapeetham, Kollam 690 525, Kerala, India., Pal A; School of Biotechnology, Amrita Vishwa Vidyapeetham, Kollam 690 525, Kerala, India., Raveendran D; Centre for Venom Informatics, University of Kerala, Thiruvananthapuram 695 581, Kerala, India; Indriyam Biologics Pvt. Ltd., SCTIMST-TIMed, BMT Wing-Poojappura, Thiruvannathapuram 695 012, Kerala, India., Nair BG; School of Biotechnology, Amrita Vishwa Vidyapeetham, Kollam 690 525, Kerala, India., Nair SS; School of Biotechnology, Amrita Vishwa Vidyapeetham, Kollam 690 525, Kerala, India. Electronic address: sudarslal@am.amrita.edu.
Jazyk: angličtina
Zdroj: International journal of biological macromolecules [Int J Biol Macromol] 2018 Oct 15; Vol. 118 (Pt B), pp. 1736-1746. Date of Electronic Publication: 2018 Jul 07.
DOI: 10.1016/j.ijbiomac.2018.07.016
Abstrakt: Hypnale hypnale (hump-nosed pit viper) is considered to be one among the medically important venomous snake species of India and Sri Lanka. In the present study, venom proteome profiling of a single Hypnale hypnale from Western Ghats of India was achieved using SDS-PAGE based protein separation followed by LC-MS/MS analysis. The identities of the proteins that were not established using the Mascot search were determined through de novo sequencing tools such as Novor followed by MS-BLAST based sequence similarity search algorithm and PEAKS proteomics software. The combined proteomics analysis revealed a total of 37 proteins belonging to nine different snake venom families, in which 7 proteins were exclusively identified through de novo strategies. The enzymatic and non-enzymatic venom protein families identified include serine proteases, metalloproteases, phospholipase A 2 , thrombin-like enzymes, phospholipase B, C-type lectins/snaclecs, disintegrins, cysteine rich secretory proteins and nerve growth factor. Among these, disintegrins, nerve growth factor, phospholipase B and cysteine rich secretory protein families were identified for the first time in HPV venom. This could possibly explain the regiospecific venom variation seen across snake species. Taken together, the venom proteome profiling on Indian Hypnale hypnale venom correlates with the clinical manifestations often seen in the envenomed victims.
(Copyright © 2018 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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