Osteolytic cancer cells induce vascular/axon guidance processes in the bone/bone marrow stroma.

Autor:	Hensel J; Urology, Department for BioMedical Research, University of Bern, Bern, Switzerland.; Department of Urology, Inselspital, Bern University Hospital, Bern, Switzerland., Wetterwald A; Urology, Department for BioMedical Research, University of Bern, Bern, Switzerland.; Department of Urology, Inselspital, Bern University Hospital, Bern, Switzerland., Temanni R; Biomedical Informatics Division, Research Branch, Sidra Medical and Research Center, Doha, Qatar., Keller I; Department for Biomedical Research, University of Bern, Bern, Switzerland.; Swiss Institute of Bioinformatics, University of Bern, Bern, Switzerland., Riether C; Tumor Immunology, Department for BioMedical Research, University of Bern, Bern, Switzerland.; Department of Medical Oncology, Inselspital, Bern University Hospital, Bern, Switzerland., van der Pluijm G; Department of Urology, Leiden University Medical Centre, Leiden, Netherlands., Cecchini MG; Urology, Department for BioMedical Research, University of Bern, Bern, Switzerland.; Department of Urology, Inselspital, Bern University Hospital, Bern, Switzerland., Thalmann GN; Urology, Department for BioMedical Research, University of Bern, Bern, Switzerland.; Department of Urology, Inselspital, Bern University Hospital, Bern, Switzerland.
Jazyk:	angličtina
Zdroj:	Oncotarget [Oncotarget] 2018 Jun 22; Vol. 9 (48), pp. 28877-28896. Date of Electronic Publication: 2018 Jun 22 (Print Publication: 2018).
DOI:	10.18632/oncotarget.25608
Abstrakt:	Prostate and breast cancers frequently metastasize to bone. The physiological bone homeostasis is perturbed once cancer cells proliferate at the bone metastatic site. Tumors are complex structures consisting of cancer cells and numerous stroma cells. In this study, we show that osteolytic cancer cells (PC-3 and MDA-MB231) induce transcriptome changes in the bone/bone marrow microenvironment (stroma). This stroma transcriptome differs from the previously reported stroma transcriptome of osteoinductive cancer cells (VCaP). While the biological process "angiogenesis/vasculogenesis" is enriched in both transcriptomes, the "vascular/axon guidance" process is a unique process that characterizes the osteolytic stroma. In osteolytic bone metastasis, angiogenesis is denoted by vessel morphology and marker expression specific for arteries/arterioles. Interestingly, intra-tumoral neurite-like structures were in proximity to arteries. Additionally, we found that increased numbers of mesenchymal stem cells and vascular smooth muscle cells, expressing osteolytic cytokines and inhibitors of bone formation, contribute to the osteolytic bone phenotype. Osteoinductive and osteolytic cancer cells induce different types of vessels, representing functionally different hematopoietic stem cell niches. This finding suggests different growth requirements of osteolytic and osteoinductive cancer cells and the need for a differential anti-angiogenic strategy to inhibit tumor growth in osteolytic and osteoblastic bone metastasis. Competing Interests: CONFLICTS OF INTEREST We have no conflicts of interests to declare.
Databáze:	MEDLINE
Externí odkaz:	Zobrazit plný text záznamu