Modulatory effects of taurine on metabolic and oxidative stress parameters in a mice model of muscle overuse.

Autor: Thirupathi A; Laboratory of Exercise Biochemistry and Physiology, Graduate Program in Health Sciences, Health Sciences Unit, Universidade do Extremo Sul Catarinense, Criciúma, Santa Catarina, Brazil., Freitas S; Laboratory of Exercise Biochemistry and Physiology, Graduate Program in Health Sciences, Health Sciences Unit, Universidade do Extremo Sul Catarinense, Criciúma, Santa Catarina, Brazil., Sorato HR; Laboratory of Exercise Biochemistry and Physiology, Graduate Program in Health Sciences, Health Sciences Unit, Universidade do Extremo Sul Catarinense, Criciúma, Santa Catarina, Brazil., Pedroso GS; Laboratory of Exercise Biochemistry and Physiology, Graduate Program in Health Sciences, Health Sciences Unit, Universidade do Extremo Sul Catarinense, Criciúma, Santa Catarina, Brazil., Effting PS; Laboratory of Exercise Biochemistry and Physiology, Graduate Program in Health Sciences, Health Sciences Unit, Universidade do Extremo Sul Catarinense, Criciúma, Santa Catarina, Brazil., Damiani AP; Laboratory of Molecular and Cellular Biology, Graduate Program in Health Sciences, Health Sciences Unit, Universidade do Extremo Sul Catarinense, Criciúma, Santa Catarina, Brazil., Andrade VM; Laboratory of Molecular and Cellular Biology, Graduate Program in Health Sciences, Health Sciences Unit, Universidade do Extremo Sul Catarinense, Criciúma, Santa Catarina, Brazil., Nesi RT; Laboratory of Exercise Biochemistry and Physiology, Graduate Program in Health Sciences, Health Sciences Unit, Universidade do Extremo Sul Catarinense, Criciúma, Santa Catarina, Brazil., Gupta RC; Nagaland University, Nagaland, India., Muller AP; Laboratory of Exercise Biochemistry and Physiology, Graduate Program in Health Sciences, Health Sciences Unit, Universidade do Extremo Sul Catarinense, Criciúma, Santa Catarina, Brazil., Pinho RA; Laboratory of Exercise Biochemistry in Health, School of Medicine, Graduate Program in Health Science, Pontifícia Universidade Católica do Paraná, Curitiba, Paraná, Brazil. Electronic address: Rapinho12@gmail.com.
Jazyk: angličtina
Zdroj: Nutrition (Burbank, Los Angeles County, Calif.) [Nutrition] 2018 Oct; Vol. 54, pp. 158-164. Date of Electronic Publication: 2018 Apr 25.
DOI: 10.1016/j.nut.2018.03.058
Abstrakt: Objective: The aim of this study was to investigate the regulatory effects of taurine on the biochemical parameters of muscle injury by overuse.
Methods: Male Swiss mice were divided into four groups: control (Ctrl), overuse (Ov), taurine (Tau), and overuse plus taurine (OvTau). High-intensity exercise sessions were administered for 21 d with concomitant subcutaneous injections of taurine (150 mg/kg). The mice were then sacrificed. The quadriceps muscles were surgically removed for subsequent histologic analysis and evaluation of mitochondrial function, oxidative stress parameters, tissue repair, and DNA damage markers.
Results: The Ov group showed significant differences compared with the Ctrl group (all P <0.05). The fiber area decreased by 49.34%, whereas the centralized nuclei contents (Ctrl = 1.33%; Ov = 28.67%), membrane potential (Ctrl suc  = 179.05 arbitrary fluorescence units (AFUs), Ctrl suc+ADP  = 198.11 AFUs; Ov suc  = 482.95 AFUs, Ov suc+ADP  = 461.6 AFUs), complex I activity (Ctrl = 20.45 nmol ⋅ min ⋅ mg protein, Ov = 45.25 nmol ⋅ min ⋅ mg protein), hydrogen peroxide (Ctrl suc  = 1.08 relative fluorescence unit (RFU) ⋅ sec ⋅ mg protein, Ctrl suc+ADP  = 0.23 RFU ⋅ sec ⋅ mg protein; Ov suc  = 5.02 RFU ⋅ sec ⋅ mg protein, Ov suc+ADP  = 0.26 RFU ⋅ sec ⋅ mg protein) and malondialdehyde (Ctrl = 0.03 nmol ⋅ mg ⋅ protein, Ov = 0.06 nmol ⋅ mg ⋅ protein) levels, and DNA damage (Ctrl freq  = 7.17%, Ov freq  = 31.17%; Ctrl index  = 4.17, Ov index  = 72.5) were increased. Taurine administration reduced the number of centralized nuclei (OvTau = 5%), hydrogen peroxide levels (OvTau suc  = 2.81 RFU ⋅ sec ⋅ mg protein, OvTaus suc+ADP  = 1.54 RFU ⋅ sec ⋅ mg protein), membrane potential (OvTau suc  = 220.18 AFUs, OvTaus suc+ADP  = 235.28 AFUs), lipid peroxidation (OvTau = 0.02 nmol/mg protein), and DNA damage (OvTau freq  = 21.33%, OvTau index  = 47.83) and increased the fiber area by 54% (all P <0.05).
Conclusion: Taken together, these data suggest that taurine supplementation modulates various cellular remodeling parameters after overuse-induced muscle damage, and that these positive effects may be related to its antioxidant capacity.
(Copyright © 2018 Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
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