Prevalence and molecular features of ESBL/pAmpC-producing Enterobacteriaceae in healthy and diseased companion animals in Brazil.

Autor: Melo LC; Department of Microbiology, Institute of Biomedical Sciences, Universidade de São Paulo, São Paulo, Brazil; Unité Antibiorésistance et Virulence Bactériennes, ANSES Laboratoire de Lyon - Université Claude Bernard Lyon 1, Lyon, France., Oresco C; Department of Microbiology, Institute of Biomedical Sciences, Universidade de São Paulo, São Paulo, Brazil., Leigue L; Department of Microbiology, Institute of Biomedical Sciences, Universidade de São Paulo, São Paulo, Brazil., Netto HM; Center of Zoonosis Control, São Paulo, Brazil., Melville PA; Department of Preventive Veterinary Medicine and Animal Health, Faculty of Veterinary Medicine, Universidade de São Paulo, São Paulo, Brazil., Benites NR; Department of Preventive Veterinary Medicine and Animal Health, Faculty of Veterinary Medicine, Universidade de São Paulo, São Paulo, Brazil., Saras E; Unité Antibiorésistance et Virulence Bactériennes, ANSES Laboratoire de Lyon - Université Claude Bernard Lyon 1, Lyon, France., Haenni M; Unité Antibiorésistance et Virulence Bactériennes, ANSES Laboratoire de Lyon - Université Claude Bernard Lyon 1, Lyon, France. Electronic address: marisa.haenni@anses.fr., Lincopan N; Department of Microbiology, Institute of Biomedical Sciences, Universidade de São Paulo, São Paulo, Brazil., Madec JY; Unité Antibiorésistance et Virulence Bactériennes, ANSES Laboratoire de Lyon - Université Claude Bernard Lyon 1, Lyon, France.
Jazyk: angličtina
Zdroj: Veterinary microbiology [Vet Microbiol] 2018 Jul; Vol. 221, pp. 59-66. Date of Electronic Publication: 2018 May 30.
DOI: 10.1016/j.vetmic.2018.05.017
Abstrakt: Extended-spectrum beta-lactamase (ESBL)- and plasmid-mediated AmpC (pAmpC)-carrying Enterobacteriaceae have widely disseminated in human, animal and environmental reservoirs. Pets have been recognized as a source of ESBL/pAmpC worldwide, and are possibly also a source of human contamination. The aim of this study was to document to what extent cats and dogs may act as a driving force in the spread of ESBLs and pAmpCs in Brazil. A total of 113 healthy stray cats and dogs and 74 sick pets were sampled, and extended-spectrum cephalosporin-resistant Enterobacteriaceae (ESC-R) were detected in 28/113 (24.8%) and 8/74 (10.8%) tested animals, respectively. Different Enterobacteriaceae isolates (mostly E. coli), a large number of E. coli clones (with ST90, ST457, ST973 and ST2541 being predominant), and several ESBL/pAmpC genes and plasmids were characterized, highlighting the ability of stray and pet cats and dogs to further spread a wide range of ESC-resistance determinants. The ESBL phenotype was due to the bla CTX-M-2 and bla CTX-M-8 genes, as found in human epidemiology in Brazil, but bla CTX-M-9 and bla CTX-M-15 were also identified. The pAmpC phenotype was systematically due to the presence of the bla CMY-2 gene, mostly carried by IncI1 ST12 plasmids. Our results showed that pets can be considered a significant reservoir of multidrug-resistant bacteria in Brazil. This is especially true for healthy stray dogs that displayed the highest prevalence (24.8%) of ESBLs/pAmpC resistance determinants, which can then be further spread both to the environment and to other animals or humans by contact.
(Copyright © 2018 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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