Characterization of two related Erwinia myoviruses that are distant relatives of the PhiKZ-like Jumbo phages.

Autor: Arens DK; Microbiology and Molecular Biology Department, Brigham Young University, Provo, Utah, United States of America., Brady TS; Microbiology and Molecular Biology Department, Brigham Young University, Provo, Utah, United States of America., Carter JL; Microbiology and Molecular Biology Department, Brigham Young University, Provo, Utah, United States of America., Pape JA; Microbiology and Molecular Biology Department, Brigham Young University, Provo, Utah, United States of America., Robinson DM; Microbiology and Molecular Biology Department, Brigham Young University, Provo, Utah, United States of America., Russell KA; Microbiology and Molecular Biology Department, Brigham Young University, Provo, Utah, United States of America., Staley LA; Microbiology and Molecular Biology Department, Brigham Young University, Provo, Utah, United States of America., Stettler JM; Plant and Wildlife Sciences Department, Brigham Young University, Provo, Utah, United States of America., Tateoka OB; Microbiology and Molecular Biology Department, Brigham Young University, Provo, Utah, United States of America., Townsend MH; Microbiology and Molecular Biology Department, Brigham Young University, Provo, Utah, United States of America., Whitley KV; Microbiology and Molecular Biology Department, Brigham Young University, Provo, Utah, United States of America., Wienclaw TM; Microbiology and Molecular Biology Department, Brigham Young University, Provo, Utah, United States of America., Williamson TL; Plant and Wildlife Sciences Department, Brigham Young University, Provo, Utah, United States of America., Johnson SM; Microbiology and Molecular Biology Department, Brigham Young University, Provo, Utah, United States of America., Grose JH; Microbiology and Molecular Biology Department, Brigham Young University, Provo, Utah, United States of America.
Jazyk: angličtina
Zdroj: PloS one [PLoS One] 2018 Jul 06; Vol. 13 (7), pp. e0200202. Date of Electronic Publication: 2018 Jul 06 (Print Publication: 2018).
DOI: 10.1371/journal.pone.0200202
Abstrakt: Bacteriophages are a major force in the evolution of bacteria due to their sheer abundance as well as their ability to infect and kill their hosts and to transfer genetic material. Bacteriophages that infect the Enterobacteriaceae family are of particular interest because this bacterial family contains dangerous animal and plant pathogens. Herein we report the isolation and characterization of two jumbo myovirus Erwinia phages, RisingSun and Joad, collected from apple trees. These two genomes are nearly identical with Joad harboring two additional putative gene products. Despite mass spectrometry data that support the putative annotation, 43% of their gene products have no significant BLASTP hit. These phages are also more closely related to Pseudomonas and Vibrio phages than to published Enterobacteriaceae phages. Of the 140 gene products with a BLASTP hit, 81% and 63% of the closest hits correspond to gene products from Pseudomonas and Vibrio phages, respectively. This relatedness may reflect their ecological niche, rather than the evolutionary history of their host. Despite the presence of over 800 Enterobacteriaceae phages on NCBI, the uniqueness of these two phages highlights the diversity of Enterobacteriaceae phages still to be discovered.
Competing Interests: JHG has a licensing agreement for a bacteriophage treatment of fire blight, for which Erwinia amylovora is the causative agent. Although the private donor is affiliated with a company that has licensed other Erwinia phages for commercial use, the funds provided for this specific study were a gift and the specific phages used in this study are not commercialized. There are no patents, products in development or marketed products to declare. This does not alter our adherence to PLOS ONE policies on sharing data and materials.
Databáze: MEDLINE
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