DNA fragmentation index and human papilloma virus in males with previous assisted reproductive technology failures.

Autor: Tangal S; Department of Urology, Ufuk University School of Medicine, Ankara, Turkey., Taşçı Y; Department of Obstetrics and Gynecology, Zekai Tahir Burak Training and Research Hospital, Ankara, Turkey., Pabuçcu EG; Department of Obstetrics and Gynecology, Ufuk University School of Medicine, Ankara, Turkey., Çağlar GS; Department of Obstetrics and Gynecology, Ufuk University School of Medicine, Ankara, Turkey., Haliloğlu AH; Department of Urology, Ufuk University School of Medicine, Ankara, Turkey., Yararbaş K; Department of Genetic, Acıbadem University School of Medicine, İstanbul, Turkey.
Jazyk: angličtina
Zdroj: Turkish journal of urology [Turk J Urol] 2018 May 21; Vol. 45 (1), pp. 12-16. Date of Electronic Publication: 2018 May 21 (Print Publication: 2019).
DOI: 10.5152/tud.2018.96393
Abstrakt: Objective: This study was designed to evaluate the prevalence of Human Papilloma Virus (HPV) in semen and document the cycle outcomes in couples with previous intra-cytoplasmic sperm injection (ICSI) failures.
Material and Methods: One hundred and seventeen couples with at least two ICSI attempts were included in the study. HPV infection in semen and DNA fragmentation in samples were analyzed by commercially available kits. The percentage of spermatozoa with fragmented DNA (DNA fragmentation index: DFI) was determined during fluorescence microscopic examination as previously described. The cycle outcomes of couples with or without HPV infected male partners were recorded.
Results: According to our results, the prevalence of HPV was 7.7% in asymptomatic males with at least two previous ICSI failures. The increased DFI (>30%) was observed in 82.9% of the cases. In HPV-positive cases significantly lower number of good quality embryos were obtained. The implantation and pregnancy rates were similar in infected and non-infected males (p>0.05). The early miscarriage rate was slightly higher in HPV- positive group (33% vs. 10%, p>0.05).
Conclusion: In cases with previous ICSI failures, the prevalence of HPV infection in semen is not higher than previously reported infertile populations. The reproductive outcome might be impaired in HPV-positive semen due to lower number of good quality embryos, which needs to be clarified by further large population-based studies.
Databáze: MEDLINE
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